Proteintech, 65204-1-Ig, Anti-Human CD8 (UCHT4)

Size: 100ug / 500ug The CD8 (65204-1-Ig) by Proteintech is a Monoclonal antibody targeting CD8 in FC applications with reactivity to Human samples 65204-1-Ig targets CD8 in FC applications and shows reactivity with Human samples. Tested Applications Positive FC detected in: human PBMCs Recommended dilution Flow Cytometry (FC): FC : 0.2 ug per 10^6 cells in 100 μl suspension Background Information CD8 is a transmembrane glycoprotein composed of two disulfide-linked chains. It can be present as a homodimer of CD8α or as a heterodimer of CD8α and CD8β (PMID: 3264320; 8253791). CD8 is found on most thymocytes. The majority of class I-restricted T cells express mostly the CD8αβ heterodimer while CD8αα homodimers alone have been found on some gut intraepithelial T cells , on some T cell receptor (TCR) γδ T cells and on NK cells (PMID: 2111591; 1831127; 8420975). CD8 acts as a co-receptor that binds to MHC class-I and participates in cytotoxic T cell activation (PMID: 8499079). During T cell development, CD8 is required for positive selection of CD4-/CD8+ T cells (PMID: 1968084). Specification Tested Reactivity: Human Host / Isotype: Mouse / IgG2a Class: Monoclonal Type: Antibody Immunogen: Thymocytes and Sézary T cells Predict reactive species Full Name: CD8a molecule Calculated Molecular Weight: 235 aa, 26 kDa GenBank Accession Number: BC025715 Gene Symbol: CD8A Gene ID (NCBI): 925 ENSEMBL Gene ID: ENSG00000153563 RRID: AB_2881486 Conjugate: Unconjugated Form: Liquid Purification Method: Protein A purification UNIPROT ID: P01732 Storage Buffer: PBS with 0.09% sodium azide, pH 7.3. Storage Conditions: Store at 2-8°C. Stable for one year after shipment.