Proteintech, 65214-1-Ig, Anti-Mouse PLVAP (MECA-32)

Size: 100ug / 500ug The PLVAP (65214-1-Ig) by Proteintech is a Monoclonal antibody targeting PLVAP in IF-P, IF-Fro, FC applications with reactivity to mouse samples 65214-1-Ig targets PLVAP in IF-P, IF-Fro, FC applications and shows reactivity with mouse samples. Tested Applications Positive IF-P detected in: mouse small intestine tissue Positive IF-Fro detected in: mouse small intestine tissue Positive FC detected in: bEnd.3 cells Recommended dilution Immunofluorescence (IF)-P: IF-P : 1:125-1:500 Immunofluorescence (IF)-FRO: IF-FRO : 1:125-1:500 Flow Cytometry (FC): FC : 0.5 ug per 10^6 cells in 100 μl suspension Background Information Plasmalemma vesicle-associated protein (PLVAP), also known as PV-1, PAL-E and MECA-32 antigen, is an endothelial cell-specific type II membrane N-glycosylated glycoprotein with a molecular weight of approximately 50-65 kDa, that forms homodimers in situ (PMID: 7626790; 30231925). The MECA-32 antibody, derived from rats immunized with murine lymph node stroma, shows high specificity for mouse endothelium in both embryonic and mature tissues (PMID: 2683264; 7626790). It recognizes most endothelial cells in the embryonic and in the adult mouse, with the exception of the brain, skeletal, and cardiac muscle, where the MECA-32 antigen has a more restricted distribution. In skeletal and cardiac muscle only small arterioles and venules express the MECA-32 antigen, while in the brain its expression is negatively correlated with the differentiation of the vasculature to form the blood brain barrier (PMID: 7626790). Specification Tested Reactivity: mouse Host / Isotype: Rat / IgG2a, kappa Class: Monoclonal Type: Antibody Immunogen: Mouse lymph node stromal cells Predict reactive species Full Name: plasmalemma vesicle associated protein GenBank Accession Number: BC013517 Gene Symbol: Plvap Gene ID (NCBI): 84094 RRID: AB_3084905 Conjugate: Unconjugated Form: Liquid Purification Method: Protein A purification UNIPROT ID: Q91VC4 Storage Buffer: PBS with 0.09% sodium azide, pH 7.3. Storage Conditions: Store at 2-8°C. Stable for one year after shipment.