Proteintech, 65226-1-Ig, Anti-Human CD45RA (F8-11-13)

Size: 100ug / 500ug The CD45RA (65226-1-Ig) by Proteintech is a Monoclonal antibody targeting CD45RA in IHC, IF-P, FC applications with reactivity to human samples 65226-1-Ig targets CD45RA in IHC, IF-P, FC applications and shows reactivity with human samples. Tested Applications Positive IHC detected in: human tonsillitis tissueNote: suggested antigen retrieval withTE buffer pH 9.0;(*) Alternatively, antigen retrieval may be performed withcitrate buffer pH 6.0 Positive IF-P detected in: human tonsillitis tissue Positive FC detected in: human PBMCs Recommended dilution Immunohistochemistry (IHC): IHC : 1:200-1:800 Immunofluorescence (IF)-P: IF-P : 1:1000-1:4000 Flow Cytometry (FC): FC : 0.2 ug per 10^6 cells in 100 μl suspension Background Information CD45, also known as protein tyrosine phosphatase, receptor type C, is a type I transmembrane protein expressed on the surface of all haematopoietic cells with the exception of erythrocytes and platelets (PMID: 3489673; 28615666). CD45 is a pan-haematopoietic cell marker and has been shown to be essential for T- and B-cell activation and signalling (PMID: 9429890; 16378097). CD45 exists as multiple isoforms due to alternative splicing of three exons (4, 5, and 6, designated A, B, and C) in the extracellular domain (PMID: 12414720). CD45RA is expressed on naïve T cells, B cells, and monocytes (PMID: 1830500; 14687231). Specification Tested Reactivity: human Host / Isotype: Mouse / IgG1, kappa Class: Monoclonal Type: Antibody Immunogen: Human T lymphocytes Predict reactive species Full Name: protein tyrosine phosphatase, receptor type, C Calculated Molecular Weight: 1304 aa, 147 kDa GenBank Accession Number: BC014239 Gene Symbol: CD45 Gene ID (NCBI): 5788 ENSEMBL Gene ID: ENSG00000081237 RRID: AB_3084907 Conjugate: Unconjugated Form: Liquid Purification Method: Protein G purification UNIPROT ID: P08575 Storage Buffer: PBS with 0.09% sodium azide, pH 7.3. Storage Conditions: Store at 2-8°C. Stable for one year after shipment.