Proteintech, 65241-1-Ig, Anti-Mouse E-cadherin (DECMA-1)

Size: 100ug / 500ug The E-cadherin (65241-1-Ig) by Proteintech is a Monoclonal antibody targeting E-cadherin in IF-P, FC applications with reactivity to human, mouse, canine samples 65241-1-Ig targets E-cadherin in IF-P, FC applications and shows reactivity with human, mouse, canine samples. Tested Applications Positive IF-P detected in: mouse colon tissue Positive FC detected in: MDCK cells Recommended dilution Immunofluorescence (IF)-P: IF-P : 1:625-1:2500 Flow Cytometry (FC): FC : 0.2 ug per 10^6 cells in 100 μl suspension Background Information Cadherins are a family of transmembrane glycoproteins that mediate calcium-dependent cell-cell adhesion and play an important role in the maintenance of normal tissue architecture. E-cadherin (epithelial cadherin), also known as CDH1 (cadherin 1) or CAM 120/80, is a classical member of the cadherin superfamily which also include N-, P-, R-, and B-cadherins. It has been regarded as a marker for spermatogonial stem cells in mice(PMID:23509752). E-cadherin is expressed on the cell surface in most epithelial tissues. The extracellular region of E-cadherin establishes calcium-dependent homophilic trans binding, providing specific interaction with adjacent cells, while the cytoplasmic domain is connected to the actin cytoskeleton through the interaction with p120-, α-, β-, and γ-catenin (plakoglobin). E-cadherin is important in the maintenance of the epithelial integrity, and is involved in mechanisms regulating proliferation, differentiation, and survival of epithelial cell. E-cadherin may also play a role in tumorigenesis. It is considered to be an invasion suppressor protein and its loss is an indicator of high tumor aggressiveness. Specification Tested Reactivity: human, mouse, canine Host / Isotype: Rat / IgG1, kappa Class: Monoclonal Type: Antibody Immunogen: Mouse embryonal carcinoma cell line PCC4 Aza RI Predict reactive species Full Name: cadherin 1 GenBank Accession Number: BC098501 Gene Symbol: E-cadherin Gene ID (NCBI): 12550 RRID: AB_2935150 Conjugate: Unconjugated Form: Liquid Purification Method: Affinity purification UNIPROT ID: P09803 Storage Buffer: PBS with 0.09% sodium azide, pH 7.3. Storage Conditions: Store at 2-8°C. Stable for one year after shipment.