Proteintech, 65254-1-Ig, Anti-Human CD35 (E11)

Size: 100ug / 500ug The CD35 (65254-1-Ig) by Proteintech is a Monoclonal antibody targeting CD35 in FC applications with reactivity to human samples 65254-1-Ig targets CD35 in FC applications and shows reactivity with human samples. Tested Applications Positive FC detected in: human PBMCs Recommended dilution Flow Cytometry (FC): FC : 0.2 ug per 10^6 cells in 100 μl suspension Background Information CD35, also known as complement receptor type 1 (CR1) or C3b/C4b receptor, is a single-chain glycoprotein consisting of 30 repeating homologous protein domains known as short consensus repeats (~60 amino acids each) followed by transmembrane and cytoplasmic domains (PMID: 8765013). CD35 is variably expressed by granulocytes, monocytes, B cells, some T cells, erythrocytes, follicular dendritic cells, Langerhans cells, and glomerular podocytes (PMID: 19004497). CD35 acts as a receptor for C3b and C4b and plays important roles in the regulation of the complement cascade and clearance of immune complexes. Specification Tested Reactivity: human Host / Isotype: Mouse / IgG1, kappa Class: Monoclonal Type: Antibody Immunogen: Human CD35 from Acute monocytic leukemia cells and normal blood monocytes Predict reactive species Full Name: complement component (3b/4b) receptor 1 (Knops blood group) Calculated Molecular Weight: 224 kDa GenBank Accession Number: NM_000573 Gene Symbol: CR1 Gene ID (NCBI): 1378 ENSEMBL Gene ID: ENSG00000203710 RRID: AB_3084912 Conjugate: Unconjugated Form: Liquid Purification Method: Affinity purification UNIPROT ID: P17927 Storage Buffer: PBS with 0.09% sodium azide, pH 7.3. Storage Conditions: Store at 2-8°C. Stable for one year after shipment.