Proteintech, 65256-1-Ig, Anti-Human IL3RA/CD123 (HI12H7)

Size: 100ug / 500ug The IL3RA/CD123 (65256-1-Ig) by Proteintech is a Monoclonal antibody targeting IL3RA/CD123 in FC applications with reactivity to human samples 65256-1-Ig targets IL3RA/CD123 in FC applications and shows reactivity with human samples. Tested Applications Positive FC detected in: human PBMCs Recommended dilution Flow Cytometry (FC): FC : 0.2 ug per 10^6 cells in 100 μl suspension Background Information CD123, also referred to as interleukin-3 receptor subunit alpha (IL-3Rα), belongs to the cytokine receptor family. It is a glycoprotein containing an extracellular domain, involving a predicted Ig-like domain, two FnIII domains, a transmembrane domain and an intracellular domain (PMID: 24513123). CD123 is expressed on hematopoietic progenitor cells, plasmacytoid dendritic cells, basophils, monocytes, some B cells, and in a variety of hematologic malignancies (PMID: 10527461; 34855461). IL-3 initially binds to CD123 and subsequently recruits the beta chain (CD131) to form the high-affinity receptor, resulting in the activation of a series of signaling pathways including the JAK/STAT, Ras-MAPK, and phosphatidylinositol 3-kinase pathways (PMID: 29296749). IL-3R signaling regulates the proliferation, survival, and differentiation of hematopoietic cells. Specification Tested Reactivity: human Host / Isotype: Mouse / IgG1, kappa Class: Monoclonal Type: Antibody Immunogen: N/A Predict reactive species Full Name: interleukin 3 receptor, alpha (low affinity) Calculated Molecular Weight: 378 aa, 43 kDa GenBank Accession Number: BC035407 Gene Symbol: CD123 Gene ID (NCBI): 3563 ENSEMBL Gene ID: ENSG00000185291 RRID: AB_3670284 Conjugate: Unconjugated Form: Liquid Purification Method: Affinity purification UNIPROT ID: P26951 Storage Buffer: PBS with 0.09% sodium azide, pH 7.3. Storage Conditions: Store at 2-8°C. Stable for one year after shipment.