Proteintech, 65570-1-MR, Anti-Human CD3 (UCHT1) Mouse IgG2a Recombinant Antibody

Size: 100ug The CD3 (65570-1-MR) by Proteintech is a Recombinant antibody targeting CD3 in FC applications with reactivity to human samples 65570-1-MR targets CD3 in FC applications and shows reactivity with human samples. Tested Applications Positive FC detected in: human PBMCs Recommended dilution Flow Cytometry (FC): FC : 0.25 ug per 10^6 cells in a 100 µl suspension Background Information CD3 is a multimeric protein associated with the T-cell receptor (TCR) to form a complex involved in antigen recognition and signal transduction (PMID: 15885124). CD3 is composed of CD3γ, δ, ε, and ζ chains (PMID: 1826255). It is expressed by thymocytes in a developmentally regulated manner, T cells, and some NK cells (PMID: 3289580). The TCR recognizes antigens bound to major histocompatibility complex (MHC) molecules. TCR-mediated peptide-MHC recognition is transmitted to the CD3 complex, leading to the intracellular signal transduction (PMID: 11985657). Specification Tested Reactivity: human Host / Isotype: Mouse / IgG2a Class: Recombinant Type: Antibody Immunogen: Human infant thymocytes and peripheral blood lymphocytes from a Sézary Syndrome donor Predict reactive species Full Name: CD3e molecule, epsilon (CD3-TCR complex) Calculated Molecular Weight: 207 aa, 23 kDa GenBank Accession Number: BC049847 Gene Symbol: CD3 Gene ID (NCBI): 916 ENSEMBL Gene ID: ENSG00000198851 RRID: AB_3670324 Conjugate: Unconjugated Form: Liquid Purification Method: Protein A purification UNIPROT ID: P07766 Storage Buffer: PBS with 0.09% sodium azide, pH 7.3. Storage Conditions: Store at 2-8°C. Stable for one year after shipment.