Proteintech, 65684-2-MR, Anti-Mouse PD-1/CD279 (RMP1-14) Mouse IgG2a Recombinant Antibody

Size: 100ug The PD-1/CD279 (65684-2-MR) by Proteintech is a Recombinant antibody targeting PD-1/CD279 in FC, Blocking, Functional Assay applications with reactivity to mouse samples 65684-2-MR targets PD-1/CD279 in FC, Blocking, Functional Assay applications and shows reactivity with mouse samples. Tested Applications Positive Functional Assay detected in: Recombinant protein Positive FC detected in: anti-CD3/CD28 treated mouse splenocytes Positive Blocking detected in: Recombinant protein Recommended dilution FUNCTIONAL ASSAY: FUNCTIONAL ASSAY : Flow Cytometry (FC): FC : 0.25 ug per 10^6 cells in a 100 µl suspension BLOCKING: BLOCKING : Background Information Programmed cell death 1 (PD-1, also known as CD279) is an immunoinhibitory receptor that belongs to the CD28/CTLA-4 subfamily of the Ig superfamily. It is a 288 amino acid (aa) type I transmembrane protein composed of one Ig superfamily domain, a stalk, a transmembrane domain, and an intracellular domain containing an immunoreceptor tyrosine-based inhibitory motif (ITIM) as well as an immunoreceptor tyrosine-based switch motif (ITSM) (PMID: 18173375). PD-1 is expressed during thymic development and is induced in a variety of hematopoietic cells in the periphery by antigen receptor signaling and cytokines (PMID: 20636820). Engagement of PD-1 by its ligands PD-L1 or PD-L2 transduces a signal that inhibits T-cell proliferation, cytokine production, and cytolytic function (PMID: 19426218). It is critical for the regulation of T cell function during immunity and tolerance. Blockade of PD-1 can overcome immune resistance and also has been shown to have antitumor activity (PMID: 22658127; 23169436). RMP1-14 is a monoclonal antibody that targets the murine PD-1 protein and can be used in mouse models to block the reaction between PD-1 and PD-L1/PD-L2 (PMID: 14764726; 31832706). Specification Tested Reactivity: mouse Host / Isotype: Mouse / IgG2a Class: Recombinant Type: Antibody Immunogen: Mouse PD-1 transfected BHK cells Predict reactive species Full Name: programmed cell death 1 GenBank Accession Number: BC119179 Gene Symbol: PD-1 Gene ID (NCBI): 18566 Conjugate: Unconjugated Form: Liquid Purification Method: Protein A purification Storage Buffer: PBS with 0.09% sodium azide, pH 7.3. Storage Conditions: Store at 2-8°C. Stable for one year after shipment.