Proteintech, 66011-1-Ig, SEC5/EXOC2 Monoclonal antibody

Size: 20ul / 150ul The SEC5/EXOC2 (66011-1-Ig) by Proteintech is a Monoclonal antibody targeting SEC5/EXOC2 in WB, IHC, IF/ICC, IP, ELISA applications with reactivity to human, mouse, pig, rat samples 66011-1-Ig targets SEC5/EXOC2 in WB, IHC, IF/ICC, IP, ELISA applications and shows reactivity with human, mouse, pig, rat samples. Tested Applications Positive WB detected in: mouse brain tissue, pig brain tissue, human brain tissue, pig cerebellum tissue, rat brain tissue, rat cerebellum tissue, HEK-293 cells Positive IP detected in: mouse brain tissue Positive IHC detected in: human breast cancer tissue, human liver cancer tissueNote: suggested antigen retrieval withTE buffer pH 9.0;(*) Alternatively, antigen retrieval may be performed withcitrate buffer pH 6.0 Positive IF/ICC detected in: HeLa cells, MDA-MB-231 cells Recommended dilution Western Blot (WB): WB : 1:2500-1:10000 Immunoprecipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate Immunohistochemistry (IHC): IHC : 1:200-1:800 Immunofluorescence (IF)/ICC: IF/ICC : 1:200-1:800 Background Information EXOC2 (exocyst complex component 2), also known as SEC5 and SEC5L1, is a component of the exocyst complex, and is required to mediate RalB-dependent survival signals in transformed cells. The exocyst complex, composed of eight evolutionarily conserved subunits (SEC3, SEC5, SEC6, SEC8, SEC10, SEC15, EXO70, and EXO84), is involved in tethering post-Golgi secretory vesicles to specific plasma membrane domains. The gene of EXOC2 maps to chromosome 6p25.3, and encodes a 924-amino acid protein with an experimentally determined molecular mass of 95-100 kDa. EXOC2 mRNA is widely expressed with highest levels in brain and placenta. Specification Tested Reactivity: human, mouse, pig, rat Cited Reactivity: human, mouse Host / Isotype: Mouse / IgG2b Class: Monoclonal Type: Antibody Immunogen: CatNo: Ag17866 Product name: Recombinant human SEC5 protein Source: e coli. -derived, PET28a Tag: 6*His Domain: 456-806 aa of BC016918 Sequence: TIQDLILDLRVRCVMATLQHTAEEIKRLAEKEDWIVDNEGLTSLPCQFEQCIVCSLQSLKGVLECKPGEASVFQQPKTQEEVCQLSINIMQVFIYCLEQLSTKPDADIDTTHLSVDVSSPDLFGSIHEDFSLTSEQRLLIVLSNCCYLERHTFLNIAEHFEKHNFQGIEKITQVSMASLKELDQRLFENYIELKADPIVGSLEPGIYAGYFDWKDCLPPTGVRNYLKEALVNIIAVHAEVFTISKELVPRVLSKVIEAVSEELSRLMQCVSSFSKNGALQARLEICALRDTVAVYLTPESKSSFKQALEALPQLSSGADKKLLEELLNKFKSSMHLQLTCFQAASSTMMKT Predict reactive species Full Name: exocyst complex component 2 Calculated Molecular Weight: 924 aa, 104 kDa Observed Molecular Weight: 100 kDa GenBank Accession Number: BC016918 Gene Symbol: SEC5 Gene ID (NCBI): 55770 RRID: AB_11042317 Conjugate: Unconjugated Form: Liquid Purification Method: Protein A purification UNIPROT ID: Q96KP1 Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20 o C storage. 20ul sizes contain 0.1% BSA.