Proteintech, 66016-1-Ig, Transketolase Monoclonal antibody

Size: 20ul / 150ul The Transketolase (66016-1-Ig) by Proteintech is a Monoclonal antibody targeting Transketolase in WB, IHC, IF/ICC, ELISA applications with reactivity to human samples 66016-1-Ig targets Transketolase in WB, IHC, IF/ICC, ELISA applications and shows reactivity with human samples. Tested Applications Positive WB detected in: HeLa cells, HepG2 cells, human liver tissue, Jurkat cells, human brain tissue, HEK-293 cells Positive IHC detected in: human colon cancer tissue, human brain tissueNote: suggested antigen retrieval withTE buffer pH 9.0;(*) Alternatively, antigen retrieval may be performed withcitrate buffer pH 6.0 Positive IF/ICC detected in: HepG2 cells Recommended dilution Western Blot (WB): WB : 1:2000-1:10000 Immunohistochemistry (IHC): IHC : 1:1000-1:4000 Immunofluorescence (IF)/ICC: IF/ICC : 1:1000-1:4000 Background Information Transketolase is a key pentose phosphate shunt enzyme that plays an important role in the production of reducing equivalents and pentose sugars. Decreased activity of transketolase has been found in patients with Alzheimer's disease and thiamine deficiency. In addition, as an important biocatalyst in stereo-specific carbon-carbon bond synthesis, transketolase has been widely used in industry to produce aromatic products. Specification Tested Reactivity: human Cited Reactivity: human Host / Isotype: Mouse / IgG2b Class: Monoclonal Type: Antibody Immunogen: CatNo: Ag16779 Product name: Recombinant human TKT protein Source: e coli. -derived, PET28a Tag: 6*His Domain: 274-623 aa of BC009970 Sequence: IYSQIQSKKKILATPPQEDAPSVDIANIRMPSLPSYKVGDKIATRKAYGQALAKLGHASDRIIALDGDTKNSTFSEIFKKEHPDRFIECYIAEQNMVSIAVGCATRNRTVPFCSTFAAFFTRAFDQIRMAAISESNINLCGSHCGVSIGEDGPSQMALEDLAMFRSVPTSTVFYPSDGVATEKAVELAANTKGICFIRTSRPENAIIYNNNEDFQVGQAKVVLKSKDDQVTVIGAGVTLHEALAAAELLKKEKINIRVLDPFTIKPLDRKLILDSARATKGRILTVEDHYYEGGIGEAVSSAVVGEPGITVTHLAVNRVPRSGKPAELLKMFGIDRDAIAQAVRGLITKA Predict reactive species Full Name: transketolase Calculated Molecular Weight: 68 kDa Observed Molecular Weight: 68 kDa GenBank Accession Number: BC009970 Gene Symbol: Transketolase Gene ID (NCBI): 7086 RRID: AB_11064729 Conjugate: Unconjugated Form: Liquid Purification Method: Protein A purification UNIPROT ID: P29401 Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20 o C storage. 20ul sizes contain 0.1% BSA.