Proteintech, 66021-1-Ig, CDC6 Monoclonal antibody

Size: 20ul / 150ul The CDC6 (66021-1-Ig) by Proteintech is a Monoclonal antibody targeting CDC6 in WB, IF/ICC, ELISA applications with reactivity to human, mouse, rat samples 66021-1-Ig targets CDC6 in WB, IF/ICC, ELISA applications and shows reactivity with human, mouse, rat samples. Tested Applications Positive WB detected in: HeLa cells, NIH/3T3 cells, ROS1728 cells, HEK-293T cells, Jurkat cell, Raji cells Positive IF/ICC detected in: sodium arsenite treated HeLa cells Recommended dilution Western Blot (WB): WB : 1:2000-1:16000 Immunofluorescence (IF)/ICC: IF/ICC : 1:400-1:1600 Background Information CDC6, also named as CDC18L and HsCDC18, belongs to the CDC6/cdc18 family. It is involved in the initiation of DNA replication and functions as a regulator at the early steps of DNA replication. It also participates in checkpoint controls that ensure DNA replication is completed before mitosis is initiated. It localizes in cell nucleus during cell cyle G1, but translocates to the cytoplasm at the start of S phase. The subcellular translocation of this protein during cell cyle is regulated through its phosphorylation by Cdks. Defects in CDC6 are the cause of Meier-Gorlin syndrome type 5 (MGORS5). MGORS5 is a syndrome characterized by bilateral microtia, aplasia/hypoplasia of the patellae, and severe intrauterine and postnatal growth retardation with short stature and poor weight gain. This antibody is a mouse monoclonal antibody raised against residues near the C terminus of human CDC6. Specification Tested Reactivity: human, mouse, rat Cited Reactivity: human, mouse Host / Isotype: Mouse / IgG1 Class: Monoclonal Type: Antibody Immunogen: CatNo: Ag17958 Product name: Recombinant human CDC6 protein Source: e coli. -derived, PET28a Tag: 6*His Domain: 217-560 aa of BC025232 Sequence: QDLKKELKGFKTIMLNCMSLRTAQAVFPAIAQEICQEEVSRPAGKDMMRKLEKHMTAEKGPMIVLVLDEMDQLDSKGQDVLYTLFEWPWLSNSHLVLIGIANTLDLTDRILPRLQAREKCKPQLLNFPPYTRNQIVTILQDRLNQVSRDQVLDNAAVQFCARKVSAVSGDVRKALDVCRRAIEIVESDVKSQTILKPLSECKSPSEPLIPKRVGLIHISQVISEIDGNRMTLSQEGAQDSFPLQQKILVCSLMLLIRQLKIKEVTLGKLYEAYSKVCRKQQVAAVDQSECLSLSGLLEARGILGLKRNKETRLTKVFFKIEEKEIEHALKDKALIGNILATGLP Predict reactive species Full Name: cell division cycle 6 homolog (S. cerevisiae) Calculated Molecular Weight: 560 aa, 63 kDa Observed Molecular Weight: 65 kDa GenBank Accession Number: BC025232 Gene Symbol: CDC6 Gene ID (NCBI): 990 RRID: AB_2881493 Conjugate: Unconjugated Form: Liquid Purification Method: Protein G purification UNIPROT ID: Q99741 Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20 o C storage. 20ul sizes contain 0.1% BSA.