Proteintech, 66034-1-Ig, TTF1/NKX2-1 Monoclonal antibody

Size: 20ul / 150ul The TTF1/NKX2-1 (66034-1-Ig) by Proteintech is a Monoclonal antibody targeting TTF1/NKX2-1 in WB, IHC, IF-P, ELISA applications with reactivity to human, pig samples 66034-1-Ig targets TTF1/NKX2-1 in WB, IHC, IF-P, ELISA applications and shows reactivity with human, pig samples. Tested Applications Positive WB detected in: HepG2 cells Positive IHC detected in: human thyroid cancer tissue, human lung cancer tissueNote: suggested antigen retrieval withTE buffer pH 9.0;(*) Alternatively, antigen retrieval may be performed withcitrate buffer pH 6.0 Positive IF-P detected in: human lung cancer tissue Recommended dilution Western Blot (WB): WB : 1:1000-1:8000 Immunohistochemistry (IHC): IHC : 1:50-1:500 Immunofluorescence (IF)-P: IF-P : 1:200-1:800 Background Information Homeobox protein Nkx-2.1 (NKX2-1), also named Thyroid nuclear factor 1,Thyroid transcription factor 1(TTF1). It is a transcription factor that binds and activates the promoter of thyroid specific genes such as thyroglobulin, thyroperoxidase, and thyrotropin receptor. Crucial in the maintenance of the thyroid differentiation phenotype. May play a role in lung development and surfactant homeostasis. Defects in NKX2-1 are the cause of benign hereditary chorea (BHC), choreoathetosis, hypothyroidism, and neonatal respiratory distress (CHNRD). Specification Tested Reactivity: human, pig Cited Reactivity: human Host / Isotype: Mouse / IgG3 Class: Monoclonal Type: Antibody Immunogen: Peptide Predict reactive species Full Name: NK2 homeobox 1 Calculated Molecular Weight: 42 kDa Observed Molecular Weight: 41 kDa GenBank Accession Number: NM_003317 Gene Symbol: TTF1 Gene ID (NCBI): 7080 RRID: AB_11045663 Conjugate: Unconjugated Form: Liquid Purification Method: Protein A purification UNIPROT ID: P43699 Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20 o C storage. 20ul sizes contain 0.1% BSA.