Proteintech, 66045-1-Ig, IA-2/PTPRN Monoclonal antibody

Size: 20ul / 150ul The IA-2/PTPRN (66045-1-Ig) by Proteintech is a Monoclonal antibody targeting IA-2/PTPRN in WB, IHC, IF/ICC, ELISA applications with reactivity to human, pig, rabbit samples 66045-1-Ig targets IA-2/PTPRN in WB, IHC, IF/ICC, ELISA applications and shows reactivity with human, pig, rabbit samples. Tested Applications Positive WB detected in: pig brain tissue, human brain tissue, rabbit brain tissue Positive IHC detected in: human pancreas cancer tissue, human brain tissueNote: suggested antigen retrieval withTE buffer pH 9.0;(*) Alternatively, antigen retrieval may be performed withcitrate buffer pH 6.0 Positive IF/ICC detected in: HepG2 cells Recommended dilution Western Blot (WB): WB : 1:2000-1:10000 Immunohistochemistry (IHC): IHC : 1:200-1:800 Immunofluorescence (IF)/ICC: IF/ICC : 1:10-1:100 Background Information IA-2/PTPRN is a member of the protein tyrosine phosphatase (PTP) family, which contains a transmembrane region, an intracellular PTP-like domain and an extracellular N-terminus. Experiments found that IA-2/PTPRN localizes to secretory granules and is exclusively expressed in neuroendocrine cells (including pancreatic islets cell). IA-2/PTPRNwas found to be a major autoantigen in insulin-dependent diabetes mellitus and the detection of autoantibodies against IA-2/PTPRN is commonly used as a diabetes diagnosis marker. Specification Tested Reactivity: human, pig, rabbit Host / Isotype: Mouse / IgG3 Class: Monoclonal Type: Antibody Immunogen: CatNo: Ag17909 Product name: Recombinant human PTPRN protein Source: e coli. -derived, PET28a Tag: 6*His Domain: 510-811 aa of BC007713 Sequence: WCEEPAQANMDISTGHMILAYMEDHLRNRDRLAKEWQALCAYQAEPNTCATAQGEGNIKKNRHPDFLPYDHARIKLKVESSPSRSDYINASPIIEHDPRMPAYIATQGPLSHTIADFWQMVWESGCTVIVMLTPLVEDGVKQCDRYWPDEGASLYHVYEVNLVSEHIWCEDFLVRSFYLKNVQTQETRTLTQFHFLSWPAEGTPASTRPLLDFRRKVNKCYRGRSCPIIVHCSDGAGRTGTYILIDMVLNRMAKGVKEIDIAATLEHVRDQRPGLVRSKDQFEFALTAVAEEVNAILKALPQ Predict reactive species Full Name: protein tyrosine phosphatase, receptor type, N Calculated Molecular Weight: 105 kDa Observed Molecular Weight: 60-65 kDa, 106-115 kDa GenBank Accession Number: BC007713 Gene Symbol: IA-2/PTPRN Gene ID (NCBI): 5798 RRID: AB_11064603 Conjugate: Unconjugated Form: Liquid Purification Method: Protein A purification UNIPROT ID: Q16849 Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20 o C storage. 20ul sizes contain 0.1% BSA.