Proteintech, 66059-1-Ig, RBM15 Monoclonal antibody

Size: 20ul / 150ul The RBM15 (66059-1-Ig) by Proteintech is a Monoclonal antibody targeting RBM15 in WB, IHC, IF/ICC, IP, ELISA applications with reactivity to human samples 66059-1-Ig targets RBM15 in WB, IHC, IF/ICC, IP, RIP, ELISA applications and shows reactivity with human samples. Tested Applications Positive WB detected in: A549 cells, human brain tissue, HeLa cells, human heart tissue, HEK-293 cells, HEK293 cells, MCF-7 cells, LNCaP cells, Jurkat cells, K-562 cells Positive IP detected in: HEK-293 cells Positive IHC detected in: human breast cancer tissue, human liver cancer tissueNote: suggested antigen retrieval withTE buffer pH 9.0;(*) Alternatively, antigen retrieval may be performed withcitrate buffer pH 6.0 Positive IF/ICC detected in: HeLa cells Recommended dilution Western Blot (WB): WB : 1:5000-1:50000 Immunoprecipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate Immunohistochemistry (IHC): IHC : 1:200-1:800 Immunofluorescence (IF)/ICC: IF/ICC : 1:200-1:800 Background Information RNA-binding motif protein 15 (RBM15) belongs to the SPEN protein family, which have repressor function in several signaling pathway, and may bind to RNA through interaction with spliceosome components. It was first described as a 5′ translocation partner of the MAL gene in t(1,22)(p13;q13) infant acute megakaryocytic leukemia, and it functions in murine hematopoiesis through modulating Notch-induced transcriptional activation, which play crucial roles in leukemogenesis. Decreasing RBM15 levels with RNA interference could inhibit the growth and proliferation, block the cell cycle, and induce apoptosis in CML cells. Specification Tested Reactivity: human Cited Reactivity: human, mouse Host / Isotype: Mouse / IgG2a Class: Monoclonal Type: Antibody Immunogen: CatNo: Ag17970 Product name: Recombinant human RBM15 protein Source: e coli. -derived, PET28a Tag: 6*His Domain: 658-957 aa of BC006397 Sequence: SSSRDRYNSDNDRSSRLLLERPSPIRDRRGSLEKSQGDKRDRKNSASAERDRKHRTTAPTEGKSPLKKEDRSDGSAPSTSTASSKLKSPSQKQDGGTAPVASASPKLCLAWQGMLLLKNSNFPSNMHLLQGDLQVASSLLVEGSTGGKVAQLKITQRLRLDQPKLDEVTRRIKVAGPNGYAILLAVPGSSDSRSSSSSAASDTATSTQRPLRNLVSYLKQKQAAGVISLPVGGNKDKENTGVLHAFPPCEFSQQFLDSPAKALAKSEEDYLVMIIVRGFGFQIGVRYENKKRENLALTLL Predict reactive species Full Name: RNA binding motif protein 15 Calculated Molecular Weight: 105 kDa Observed Molecular Weight: 107 kDa GenBank Accession Number: BC006397 Gene Symbol: RBM15 Gene ID (NCBI): 64783 RRID: AB_11042326 Conjugate: Unconjugated Form: Liquid Purification Method: Protein A purification UNIPROT ID: Q96T37 Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20 o C storage. 20ul sizes contain 0.1% BSA.