Proteintech, 66070-1-Ig, Integrin Alpha 3 Monoclonal antibody

Size: 20ul / 150ul The Integrin Alpha 3 (66070-1-Ig) by Proteintech is a Monoclonal antibody targeting Integrin Alpha 3 in WB, IHC, ELISA applications with reactivity to human, pig, mouse samples 66070-1-Ig targets Integrin Alpha 3 in WB, IHC, IF, IP, ELISA applications and shows reactivity with human, pig, mouse samples. Tested Applications Positive WB detected in: PC-3 cells, A431 cells, A549 cells, HepG2 cells, mouse brain tissue Positive IHC detected in: human lung cancer tissueNote: suggested antigen retrieval withTE buffer pH 9.0;(*) Alternatively, antigen retrieval may be performed withcitrate buffer pH 6.0 Recommended dilution Western Blot (WB): WB : 1:500-1:2000 Immunohistochemistry (IHC): IHC : 1:2000-1:8000 Background Information ITGA3, also named as MSK18, CD49c, GAPB3 and FRP-2, belongs to the integrin alpha chain family. It is a receptor for fibronectin, laminin, collagen, epiligrin, thrombospondin and CSPG4. ITGA3 may mediate with LGALS3 the stimulation by CSPG4 of endothelial cells migration. ITGA3 is highly concentrated in epithelial cells where it strongly adheres to Laminin-5 and Laminin-5 induced rapid adhesion can be blocked by antibodies against the alpha-3 integrin subunit. It can be cleaved into heavy chain(840aa) and light chain(176aa). This antibody can recognize the heavy chain. Specification Tested Reactivity: human, pig, mouse Cited Reactivity: human, mouse, pig, rabbit Host / Isotype: Mouse / IgG2a Class: Monoclonal Type: Antibody Immunogen: CatNo: Ag17293 Product name: Recombinant human Integrin alpha-3 protein Source: e coli. -derived, PGEX-4T Tag: GST Domain: 52-376 aa of BC150190 Sequence: FGYSVALHRQTERQQRYLLLAGAPRELAVPDGYTNRTGAVYLCPLTAHKDDCERMNITVKNDPGHHIIEDMWLGVTVASQGPAGRVLVCAHRYTQVLWSGSEDQRRMVGKCYVRGNDLELDSSDDWQTYHNEMCNSNTDYLETGMCQLGTSGGFTQNTVYFGAPGAYNWKGNSYMIQRKEWDLSEYSYKDPEDQGNLYIGYTMQVGSFILHPKNITIVTGAPRHRHMGAVFLLSQEAGGDLRRRQVLEGSQVGAYFGSAIALADLNNDGWQDLLVGAPYYFERKEEVGGAIYVFMNQAGTSFPAHPSLLLHGPSGSAFGLSVASI Predict reactive species Full Name: integrin, alpha 3 (antigen CD49C, alpha 3 subunit of VLA-3 receptor) Calculated Molecular Weight: 1066 aa, 119 kDa Observed Molecular Weight: 120 kDa GenBank Accession Number: BC150190 Gene Symbol: Integrin alpha 3 Gene ID (NCBI): 3675 RRID: AB_11042765 Conjugate: Unconjugated Form: Liquid Purification Method: Protein A purification UNIPROT ID: P26006 Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20 o C storage. 20ul sizes contain 0.1% BSA.