Proteintech, 66071-1-Ig, EPLIN Monoclonal antibody

Size: 20ul / 150ul The EPLIN (66071-1-Ig) by Proteintech is a Monoclonal antibody targeting EPLIN in WB, IHC, IF/ICC, ELISA applications with reactivity to human, rat samples 66071-1-Ig targets EPLIN in WB, IHC, IF/ICC, CoIP, ELISA applications and shows reactivity with human, rat samples. Tested Applications Positive WB detected in: HeLa cells, A431 cells, COLO 320 cells, HEK-293 cells, human brain tissue, HaCaT cells, SCaBER cells, MDA-MB-231 cells, SK-BR-3 cells, HT-29 cells, pig colon tissue, rat colon tissue, HSC-T6 cells, ROS1728 cells Positive IHC detected in: human heart tissueNote: suggested antigen retrieval withTE buffer pH 9.0;(*) Alternatively, antigen retrieval may be performed withcitrate buffer pH 6.0 Positive IF/ICC detected in: Hela cells Recommended dilution Western Blot (WB): WB : 1:2000-1:10000 Immunohistochemistry (IHC): IHC : 1:20-1:200 Immunofluorescence (IF)/ICC: IF/ICC : 1:20-1:200 Background Information EPLIN is a cytoskeletal protein that is preferentially expressed in epithelial cells and is implicated in regulation of cytoskeletal dynamics and cytokinesis. In human, there are two known isoforms, EPLIN-a and -b, generated by alternative promoter usage from a single gene. EPLIN-a expression is often down-regulated in cancerous cells and tissues. This antibody recognizes both of isoforms of EPLN. Specification Tested Reactivity: human, rat Cited Reactivity: human, mouse Host / Isotype: Mouse / IgG1 Class: Monoclonal Type: Antibody Immunogen: CatNo: Ag13288 Product name: Recombinant human LIMA1 protein Source: e coli. -derived, PET28a Tag: 6*His Domain: 261-401 aa of BC001247 Sequence: KPLSPDSRASSLSESSPPKAMKFQAPARETCVECQKTVYPMERLLANQQVFHISCFRCSYCNNKLSLGTYASLHGRIYCKPHFNQLFKSKGNYDEGFGHRPHKDLWASKNENEEILERPAQLANARETPHSPGVEDAPIAK Predict reactive species Full Name: LIM domain and actin binding 1 Calculated Molecular Weight: 85 kDa Observed Molecular Weight: 90 kDa, 110 kDa GenBank Accession Number: BC001247 Gene Symbol: EPLIN Gene ID (NCBI): 51474 RRID: AB_11043341 Conjugate: Unconjugated Form: Liquid Purification Method: Protein G purification UNIPROT ID: Q9UHB6 Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20 o C storage. 20ul sizes contain 0.1% BSA.