Proteintech, 66180-1-Ig, CD71 Monoclonal antibody

Size: 20ul / 150ul The CD71 (66180-1-Ig) by Proteintech is a Monoclonal antibody targeting CD71 in WB, IHC, IF/ICC, IF-P, IP, ELISA applications with reactivity to human samples 66180-1-Ig targets CD71 in WB, IHC, IF/ICC, IF-P, IP, ELISA, Blocking assay applications and shows reactivity with human samples. Tested Applications Positive WB detected in: U-251 cells, A549 cells, HeLa cells, Raji cells, TF-1 cells, U2OS cells, A431 cells, HepG2 cells, K-562 cells Positive IP detected in: HeLa cells Positive IHC detected in: human placenta tissue, human breast cancer tissueNote: suggested antigen retrieval withTE buffer pH 9.0;(*) Alternatively, antigen retrieval may be performed withcitrate buffer pH 6.0 Positive IF-P detected in: human breast cancer tissue, MCF-7 cells Positive IF/ICC detected in: MCF-7 cells, K-562 cells Recommended dilution Western Blot (WB): WB : 1:5000-1:50000 Immunoprecipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate Immunohistochemistry (IHC): IHC : 1:2000-1:8000 Immunofluorescence (IF)-P: IF-P : 1:200-1:800 Immunofluorescence (IF)/ICC: IF/ICC : 1:200-1:800 Background Information CD71, also known as transferrin receptor protein 1 (TfR1), is a transmembrane glycoprotein composed of two disulfide-linked monomers, each of 90 kDa molecular weight. Each monomer binds one holo-transferrin molecule creating an iron-Tf-TfR complex which enters the cell by endocytosis. CD71 is present on actively proliferating cells and is essential for iron transport into proliferating cells. Specification Tested Reactivity: human Cited Reactivity: human Host / Isotype: Mouse / IgG2a Class: Monoclonal Type: Antibody Immunogen: CatNo: Ag21612 Product name: Recombinant human CD71 protein Source: e coli. -derived, PET30a Tag: 6*His Domain: 513-757 aa of BC001188 Sequence: VKHPVTGQFLYQDSNWASKVEKLTLDNAAFPFLAYSGIPAVSFCFCEDTDYPYLGTTMDTYKELIERIPELNKVARAAAEVAGQFVIKLTHDVELNLDYERYNSQLLSFVRDLNQYRADIKEMGLSLQWLYSARGDFFRATSRLTTDFGNAEKTDRFVMKKLNDRVMRVEYHFLSPYVSPKESPFRHVFWGSGSHTLPALLENLKLRKQNNGAFNETLFRNQLALATWTIQGAANALSGDVWDID Predict reactive species Full Name: transferrin receptor (p90, CD71) Calculated Molecular Weight: 85 kDa Observed Molecular Weight: 90 kDa GenBank Accession Number: BC001188 Gene Symbol: CD71 Gene ID (NCBI): 7037 ENSEMBL Gene ID: ENSG00000072274 RRID: AB_2881575 Conjugate: Unconjugated Form: Liquid Purification Method: Protein A purification UNIPROT ID: P02786 Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20 o C storage. 20ul sizes contain 0.1% BSA.