Proteintech, 66183-1-Ig, HSP70 Monoclonal antibody

Size: 20ul / 150ul The HSP70 (66183-1-Ig) by Proteintech is a Monoclonal antibody targeting HSP70 in WB, IHC, IF/ICC, FC (Intra), ELISA applications with reactivity to human, mouse, rat, yeast samples 66183-1-Ig targets HSP70 in WB, IHC, IF/ICC, FC (Intra), CoIP, ELISA applications and shows reactivity with human, mouse, rat, yeast samples. Tested Applications Positive WB detected in: LNCaP cells, HEK-293 cells, HeLa cells, Recombinant protein, whole yeast, HEK-293T cells, K-562 cells Positive IHC detected in: human hepatocellular carcinoma, human pancreas cancer tissueNote: suggested antigen retrieval withTE buffer pH 9.0;(*) Alternatively, antigen retrieval may be performed withcitrate buffer pH 6.0 Positive IF/ICC detected in: HeLa cells Positive FC (Intra) detected in: HeLa cells Recommended dilution Western Blot (WB): WB : 1:20000-1:100000 Immunohistochemistry (IHC): IHC : 1:400-1:1600 Immunofluorescence (IF)/ICC: IF/ICC : 1:400-1:1600 Flow Cytometry (FC) (INTRA): FC (INTRA) : 0.25 ug per 10^6 cells in a 100 µl suspension Background Information HSPA1A , collectively known as HSP70 (also referred to HSP72), is a stress-inducible member of heat-shock protein 70 (HSP70) proteins which are highly conserved chaperons implicated in protein folding, protein refolding, protein transport, and protein targeting. Encoded by two closely linked, intronless and stress-inducible genes, HSPA1A and HSPA1B differ by only two amino acids and are believed to be fully interchangeable proteins. HSPA1A is a cytosol nuclear protein able to translocate between cytoplasm and nucleus. Generally, HSPA1A is thought to be expressed in unstressed normal cells at low or undetectable levels. Expression of HSPA1A protein can be highly activated by various stressful stimuli. Significant up-regulation of HSPA1A has been found in various tumors. Recently it has been reported that HSPA1A can be constitutively expressed in selected cell types. HSP70 is also used as exosomal marker. This antibody well recognized the endogenous HSP70 protein in multiple cell lines. (21373891) Specification Tested Reactivity: human, mouse, rat, yeast Cited Reactivity: human, mouse, rat, pig Host / Isotype: Mouse / IgG1 Class: Monoclonal Type: Antibody Immunogen: CatNo: Ag19111 Product name: Recombinant human HSP70 protein Source: e coli. -derived, PET28a Tag: 6*His Domain: 291-641 aa of BC009322 Sequence: IDFYTSITRARFEELCSDLFRSTLEPVEKALRDAKLDKAQIHDLVLVGGSTRIPKVQKLLQDFFNGRDLNKSINPDEAVAYGAAVQAAILMGDKSENVQDLLLLDVAPLSLGLETAGGVMTALIKRNSTIPTKQTQIFTTYSDNQPGVLIQVYEGERAMTKDNNLLGRFELSGIPPAPRGVPQIEVTFDIDANGILNVTATDKSTGKANKITITNDKGRLSKEEIERMVQEAEKYKAEDEVQRERVSAKNALESYAFNMKSAVEDEGLKGKISEADKKKVLDKCQEVISWLDANTLAEKDEFEHKRKELEQVCNPIISGLYQGAGGPGPGGFGAQGPKGGSGSGPTIEEVD Predict reactive species Full Name: heat shock 70kDa protein 1A Calculated Molecular Weight: 70 kDa Observed Molecular Weight: 65-70 kDa GenBank Accession Number: BC009322 Gene Symbol: HSP70 Gene ID (NCBI): 3303 RRID: AB_2881578 Conjugate: Unconjugated Form: Liquid Purification Method: Protein G purification UNIPROT ID: P0DMV8 Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20 o C storage. 20ul sizes contain 0.1% BSA.