Proteintech, 66214-1-Ig, MYOD1 Monoclonal antibody

Size: 20ul / 150ul The MYOD1 (66214-1-Ig) by Proteintech is a Monoclonal antibody targeting MYOD1 in WB, ELISA applications with reactivity to human, mouse, rat, pig samples 66214-1-Ig targets MYOD1 in WB, IHC, IF, ELISA applications and shows reactivity with human, mouse, rat, pig samples. Tested Applications Positive WB detected in: human skeletal muscle tissue Recommended dilution Western Blot (WB): WB : 1:500-1:2000 Background Information MYOD1, also named as BHLHC1 or MYF3, is a 320 amino acid protein, which promotes the transcriptional activity of MYOD1 through its CDK9-mediated phosphorylation. This phosphorylation promotes its function in muscle differentiation. MYOD1 acts as a transcriptional activator that promotes transcription of muscle-specific target genes and plays a role in muscle differentiation. MYOD1 together with MYF5 and MYOG, co-occupies muscle-specific gene promoter core region during myogenesis. Induces fibroblasts to differentiate into myoblasts. he calculated molecular weight of MYOD1 is 34 kDa, but modified MYOD1 is about 45-47 kDa. (PMID: 12037670) Specification Tested Reactivity: human, mouse, rat, pig Cited Reactivity: human, mouse, rat, pig Host / Isotype: Mouse / IgG1 Class: Monoclonal Type: Antibody Immunogen: CatNo: Ag13512 Product name: Recombinant human MYOD1 protein Source: e coli. -derived, PGEX-4T Tag: GST Domain: 1-320 aa of BC064493 Sequence: MELLSPPLRDVDLTAPDGSLCSFATTDDFYDDPCFDSPDLRFFEDLDPRLMHVGALLKPEEHSHFPAAVHPAPGAREDEHVRAPSGHHQAGRCLLWACKACKRKTTNADRRKAATMRERRRLSKVNEAFETLKRCTSSNPNQRLPKVEILRNAIRYIEGLQALLRDQDAAPPGAAAAFYAPGPLPPGRGGEHYSGDSDASSPRSNCSDGMMDYSGPPSGARRRNCYEGAYYNEAPSEPRPGKSAAVSSLDCLSSIVERISTESPAAPALLLADVPSESPPRRQEAAAPSEGESSGDPTQSPDAAPQCPAGANPNPIYQVL Predict reactive species Full Name: myogenic differentiation 1 Calculated Molecular Weight: 320 aa, 35 kDa Observed Molecular Weight: 47 kDa GenBank Accession Number: BC064493 Gene Symbol: MYOD1 Gene ID (NCBI): 4654 RRID: AB_2881605 Conjugate: Unconjugated Form: Liquid Purification Method: Protein G purification UNIPROT ID: P15172 Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20 o C storage. 20ul sizes contain 0.1% BSA.