Proteintech, 66236-1-Ig, SALL4 Monoclonal antibody

Size: 20ul / 150ul The SALL4 (66236-1-Ig) by Proteintech is a Monoclonal antibody targeting SALL4 in WB, ELISA applications with reactivity to human samples 66236-1-Ig targets SALL4 in WB, IHC, ELISA applications and shows reactivity with human samples. Tested Applications Positive WB detected in: K-562 cells Recommended dilution Western Blot (WB): WB : 1:500-1:2000 Background Information SALL4, also named as Sal-like protein 4 or Zinc finger protein 797, Contains 7 C2H2-type zinc fingers and belongs to the sal C2H2-type zinc-finger protein family. SALL4 is constitutively expressed in acute myeloid leukemia. The constitutive expression of SALL4in mice is sufficient to induce MDS-like symptoms and transformation to AML that is transplantable. SALL4 is able to bind beta-catenin and activate the Wnt/beta-catenin signaling pathway. Sequence analysis of the larger cDNA fragment isolated revealed a single, large open-reading frame, designated as SALL4A, that started from a strong consensus initiation sequence and was expected to encode 1053 amino acids. The other splicing variant of SALL4, designated SALL4B, lacked the region corresponding to amino acids 385 to 820 of the full-length SALL4A. The putative protein encoded by SALL4B cDNA was expected to consist of 617 amino acids. Specification Tested Reactivity: human Cited Reactivity: human Host / Isotype: Mouse / IgG1 Class: Monoclonal Type: Antibody Immunogen: CatNo: Ag17480 Product name: Recombinant human SALL4 protein Source: e coli. -derived, PET28a Tag: 6*His Domain: 1-350 aa of BC111714 Sequence: MSRRKQAKPQHINSEEDQGEQQPQQQTPEFADAAPAAPAAGELGAPVNHPGNDEVASEDEATVKRLRREETHVCEKCCAEFFSISEFLEHKKNCTKNPPVLIMNDSEGPVPSEDFSGAVLSHQPTSPGSKDCHRENGGSSEDMKEKPDAESVVYLKTETALPPTPQDISYLAKGKVANTNVTLQALRGTKVAVNQRSADALPAPVPGANSIPWVLEQILCLQQQQLQQIQLTEQIRIQVNMWASHALHSSGAGADTLKTLGSHMSQQVSAAVALLSQKAGSQGLSLDALKQAKLPHANIPSATSSLSPGLAPFTLKPDGTRVLPNVMSRLPSALLPQAPGSVLFQSPFST Predict reactive species Full Name: sal-like 4 (Drosophila) Calculated Molecular Weight: 1053 aa, 112 kDa Observed Molecular Weight: 165 kDa GenBank Accession Number: BC111714 Gene Symbol: SALL4 Gene ID (NCBI): 57167 RRID: AB_2881625 Conjugate: Unconjugated Form: Liquid Purification Method: Protein G purification UNIPROT ID: Q9UJQ4 Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20 o C storage. 20ul sizes contain 0.1% BSA.