Proteintech, 66259-1-Ig, Nestin Monoclonal antibody

Size: 20ul / 150ul The Nestin (66259-1-Ig) by Proteintech is a Monoclonal antibody targeting Nestin in WB, ELISA applications with reactivity to human, mouse, rat samples 66259-1-Ig targets Nestin in WB, ELISA applications and shows reactivity with human, mouse, rat samples. Tested Applications Positive WB detected in: Neuro-2a cells, HEK-293 cells, PC-12 cells, rat cerebellum tissue Recommended dilution Western Blot (WB): WB : 1:500-1:2000 Background Information Nestin, also named as NES and Nbla00170, is a class VI intermediate filament protein expressed in stem cells of the central nervous system (CNS) but not in mature CNS cells. Its expression is used extensively as a marker for neural lineage cells derived from human embryonic stem (hES) and induced pluripotent stem (iPS) cells. Nestin is also expressed in non-neural stem cell populations such as pancreatic islet progenitors and hematopoietic progenitors. Specification Tested Reactivity: human, mouse, rat Cited Reactivity: human, mouse, rat Host / Isotype: Mouse / IgG2b Class: Monoclonal Type: Antibody Immunogen: Peptide Predict reactive species Full Name: nestin Calculated Molecular Weight: 177 kDa Observed Molecular Weight: 150-170 kDa GenBank Accession Number: NM_006617 Gene Symbol: Nestin Gene ID (NCBI): 10763 RRID: AB_2756523 Conjugate: Unconjugated Form: Liquid Purification Method: Protein A purification UNIPROT ID: P48681 Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20 o C storage. 20ul sizes contain 0.1% BSA.