Proteintech, 66274-1-Ig, CLTCL1 Monoclonal antibody

Size: 20ul / 150ul The CLTCL1 (66274-1-Ig) by Proteintech is a Monoclonal antibody targeting CLTCL1 in WB, IHC, IF/ICC, ELISA applications with reactivity to human samples 66274-1-Ig targets CLTCL1 in WB, IHC, IF/ICC, ELISA applications and shows reactivity with human samples. Tested Applications Positive WB detected in: HeLa cells, HEK-293 cells, Jurkat cells Positive IHC detected in: human skeletal muscle tissueNote: suggested antigen retrieval withTE buffer pH 9.0;(*) Alternatively, antigen retrieval may be performed withcitrate buffer pH 6.0 Positive IF/ICC detected in: HeLa cells Recommended dilution Western Blot (WB): WB : 1:500-1:2000 Immunohistochemistry (IHC): IHC : 1:50-1:500 Immunofluorescence (IF)/ICC: IF/ICC : 1:50-1:500 Background Information CLTCL1, also named as CLH22, CLTCL and CLTD, belongs to the clathrin heavy chain family. Clathrin is the major protein of the polyhedral coat of coated pits and vesicles. Two different adapter protein complexes link the clathrin lattice either to the plasma membrane or to the trans-Golgi network. The antibody is specific to CLTCL1. It has no cross reaction to CLTC. Specification Tested Reactivity: human Host / Isotype: Mouse / IgG2a Class: Monoclonal Type: Antibody Immunogen: Peptide Predict reactive species Full Name: clathrin, heavy chain-like 1 Calculated Molecular Weight: 187 kDa Observed Molecular Weight: 180 kDa GenBank Accession Number: NM_007098 Gene Symbol: CLTCL1 Gene ID (NCBI): 8218 RRID: AB_2881657 Conjugate: Unconjugated Form: Liquid Purification Method: Protein A purification UNIPROT ID: P53675 Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20 o C storage. 20ul sizes contain 0.1% BSA.