Proteintech, 66301-1-Ig, CD107b / LAMP2 Monoclonal antibody

Size: 20ul / 150ul The CD107b / LAMP2 (66301-1-Ig) by Proteintech is a Monoclonal antibody targeting CD107b / LAMP2 in WB, IHC, ELISA applications with reactivity to human, mouse, pig samples 66301-1-Ig targets CD107b / LAMP2 in WB, IHC, IP, CoIP, ELISA applications and shows reactivity with human, mouse, pig samples. Tested Applications Positive WB detected in: L02 cells, pig liver tissue, mouse liver tissue, HEK-293 cells, HUVEC cells, Jurkat cells, HeLa cells, HepG2 cells Positive IHC detected in: human liver cancer tissue, mouse liver tissue, human lung cancer tissue, human liver tissue, human kidney tissueNote: suggested antigen retrieval withTE buffer pH 9.0;(*) Alternatively, antigen retrieval may be performed withcitrate buffer pH 6.0 Recommended dilution Western Blot (WB): WB : 1:1000-1:4000 Immunohistochemistry (IHC): IHC : 1:500-1:2000 Background Information Lysosomal-associated membrane protein 2 (LAMP2, synonyms: LAMPB, CD107b) is a member of a family of membrane glycoproteins. This glycoprotein provides selectins with carbohydrate ligands. LAMP2 may play a role in tumor cell metastasis. It may also function in the protection, maintenance, and adhesion of the lysosome. Prior to posttranslational modification, LAMP2 is a ~45 kDa polypeptide. Mature, functional LAMP2 is extensively glycosylated with a variety of different N-linked and O-linked oligosaccharides. Specification Tested Reactivity: human, mouse, pig Cited Reactivity: human, mouse, pig, chicken, bovine, sheep Host / Isotype: Mouse / IgA Class: Monoclonal Type: Antibody Immunogen: CatNo: Ag0595 Product name: Recombinant human LAMP2 protein Source: e coli. -derived, PGEX-4T Tag: GST Domain: 255-390 aa of BC002965 Sequence: NPNTTHSTGSCRSHTALLRLNSSTIKYLDFVFAVKNENRFYLKEVNISMYLVNGSVFSIANNNLSYWDAPLGSSYMCNKEQTVSVSGAFQINTFDLRVQPFNVTQGKYSTAQECSLDDDTILIPIIVGAGLSGLII Predict reactive species Full Name: lysosomal-associated membrane protein 2 Calculated Molecular Weight: 45 kDa Observed Molecular Weight: 120 kDa GenBank Accession Number: BC002965 Gene Symbol: LAMP2 Gene ID (NCBI): 3920 ENSEMBL Gene ID: ENSG00000005893 RRID: AB_2881684 Conjugate: Unconjugated Form: Liquid Purification Method: Thiophilic affinity chromatograph UNIPROT ID: P13473 Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20 o C storage. 20ul sizes contain 0.1% BSA.