Proteintech, 66335-1-Ig, NUDT21 Monoclonal antibody

Size: 20ul / 150ul The NUDT21 (66335-1-Ig) by Proteintech is a Monoclonal antibody targeting NUDT21 in WB, IHC, IF/ICC, ELISA applications with reactivity to human, mouse samples 66335-1-Ig targets NUDT21 in WB, IHC, IF/ICC, IP, RIP, ELISA applications and shows reactivity with human, mouse samples. Tested Applications Positive WB detected in: NIH/3T3 cells, HEK-293 cells, HeLa cells, MCF-7 cells Positive IHC detected in: human brain tissue, mouse brain tissue, mouse liver tissueNote: suggested antigen retrieval withTE buffer pH 9.0;(*) Alternatively, antigen retrieval may be performed withcitrate buffer pH 6.0 Positive IF/ICC detected in: HeLa cells, HepG2 cells Recommended dilution Western Blot (WB): WB : 1:1000-1:6000 Immunohistochemistry (IHC): IHC : 1:500-1:2000 Immunofluorescence (IF)/ICC: IF/ICC : 1:1000-1:4000 Background Information Cleavage and polyadenylation specific factor 5, 25 kDa (CPSF5, synonym: CFIM25) is one subunit of a cleavage factor required for 3' RNA cleavage and polyadenylation processing. The interaction of CPSF5 with the RNA is one of the earliest steps in the assembly of the 3' end processing complex and facilitates the recruitment of other processing factors. Specification Tested Reactivity: human, mouse Cited Reactivity: human Host / Isotype: Mouse / IgG1 Class: Monoclonal Type: Antibody Immunogen: CatNo: Ag12260 Product name: Recombinant human NUDT21 protein Source: e coli. -derived, PET28a Tag: 6*His Domain: 1-222 aa of BC001403 Sequence: MSVVPPNRSQTGWPRGVTQFGNKYIQQTKPLTLERTINLYPLTNYTFGTKEPLYEKDSSVAARFQRMREEFDKIGMRRTVEGVLIVHEHRLPHVLLLQLGTTFFKLPGGELNPGEDEVEGLKRLMTEILGRQDGVLQDWVIDDCIGNWWRPNFEPPQYPYIPAHITKPKEHKKLFLVQLQEKALFAVPKNYKLVAAPLFELYDNAPGYGPIISSLPQLLSRF Predict reactive species Full Name: nudix (nucleoside diphosphate linked moiety X)-type motif 21 Calculated Molecular Weight: 26 kDa Observed Molecular Weight: 27 kDa GenBank Accession Number: BC001403 Gene Symbol: NUDT21 Gene ID (NCBI): 11051 RRID: AB_2881715 Conjugate: Unconjugated Form: Liquid Purification Method: Protein G purification UNIPROT ID: O43809 Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20 o C storage. 20ul sizes contain 0.1% BSA.