Proteintech, 66346-1-Ig, ASNA1 Monoclonal antibody

Size: 20ul / 150ul The ASNA1 (66346-1-Ig) by Proteintech is a Monoclonal antibody targeting ASNA1 in WB, IHC, IF/ICC, ELISA applications with reactivity to human, mouse, rat, pig, chicken samples 66346-1-Ig targets ASNA1 in WB, IHC, IF/ICC, ELISA applications and shows reactivity with human, mouse, rat, pig, chicken samples. Tested Applications Positive WB detected in: Neuro-2a cells, fetal human brain tissue, pig brain tissue, HEK-293 cells, HeLa cells, rat brain tissue, rabbit brain tissue, mouse brain tissue, chicken brain tissue Positive IHC detected in: human lung cancer tissueNote: suggested antigen retrieval withTE buffer pH 9.0;(*) Alternatively, antigen retrieval may be performed withcitrate buffer pH 6.0 Positive IF/ICC detected in: HEK-293 cells Recommended dilution Western Blot (WB): WB : 1:5000-1:50000 Immunohistochemistry (IHC): IHC : 1:50-1:500 Immunofluorescence (IF)/ICC: IF/ICC : 1:200-1:800 Background Information ASNA1 (also known as TRC40) is a highly conserved ATPase involved in efflux of arsenite and antimonite. Reduced ASNA1 expression is associated with significant inhibition of cell growth, increased apoptosis and increased sensitivity to arsenite.Thus ASNA1 is proposed to be a target to overcome resistance to cancer chemotherapy. In addition, ASNA1 has been identified as an ER targeting factor for tail-anchored proteins in the posttranslational membrane insertion pathway. Specification Tested Reactivity: human, mouse, rat, pig, chicken Host / Isotype: Mouse / IgG1 Class: Monoclonal Type: Antibody Immunogen: CatNo: Ag7936 Product name: Recombinant human ASNA1 protein Source: e coli. -derived, PET28a Tag: 6*His Domain: 1-348 aa of BC002651 Sequence: MAAGVAGWGVEAEEFEDAPDVEPLEPTLSNIIEQRSLKWIFVGGKGGVGKTTCSCSLAVQLSKGRESVLIISTDPAHNISDAFDQKFSKVPTKVKGYDNLFAMEIDPSLGVAELPDEFFEEDNMLSMGKKMMQEAMSAFPGIDEAMSYAEVMRLVKGMNFSVVVFDTAPTGHTLRLLNFPTIVERGLGRLMQIKNQISPFISQMCNMLGLGDMNADQLASKLEETLPVIRSVSEQFKDPEQTTFICVCIAEFLSLYETERLIQELAKCKIDTHNIIVNQLVFPDPEKPCKMCEARHKIQAKYLDQMEDLYEDFHIVKLPLLPHEVRGADKVNTFSALLLEPYKPPSAQ Predict reactive species Full Name: arsA arsenite transporter, ATP-binding, homolog 1 (bacterial) Calculated Molecular Weight: 38.7 kDa Observed Molecular Weight: 37-41 kDa GenBank Accession Number: BC002651 Gene Symbol: ASNA1 Gene ID (NCBI): 439 RRID: AB_2881726 Conjugate: Unconjugated Form: Liquid Purification Method: Protein G purification UNIPROT ID: O43681 Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20 o C storage. 20ul sizes contain 0.1% BSA.