Proteintech, 66362-1-Ig, Tubulin Beta Class V Monoclonal antibody

Size: 20ul / 150ul The Tubulin Beta Class V (66362-1-Ig) by Proteintech is a Monoclonal antibody targeting Tubulin Beta Class V in WB, IHC, IF/ICC, ELISA applications with reactivity to human, mouse, Canine samples 66362-1-Ig targets Tubulin Beta Class V in WB, IHC, IF/ICC, ELISA applications and shows reactivity with human, mouse, Canine samples. Tested Applications Positive WB detected in: HeLa cells, human testis tissue, NCCIT cell, NIH/3T3 cells Positive IHC detected in: human lung cancer tissue, human breast cancer tissueNote: suggested antigen retrieval withTE buffer pH 9.0;(*) Alternatively, antigen retrieval may be performed withcitrate buffer pH 6.0 Positive IF/ICC detected in: MDCK cells Recommended dilution Western Blot (WB): WB : 1:500-1:2000 Immunohistochemistry (IHC): IHC : 1:50-1:500 Immunofluorescence (IF)/ICC: IF/ICC : 1:50-1:500 Background Information Tubulin beta class V, encoded by TUBB6, is an isotype of beta tubulin, the major component of microtubule. It is widely expressed in many tissues. In normal tissues, tubulin beta class V is focally expressed in ciliary structure, while in cancer tissues it exhibited a diffuse staining pattern. The alterations of its expression in tumors suggest tubulin beta class V may be used as a biomarker for some solid tumors. Specification Tested Reactivity: human, mouse, Canine Cited Reactivity: human, mouse, rat Host / Isotype: Mouse / IgG1 Class: Monoclonal Type: Antibody Immunogen: Peptide Predict reactive species Full Name: tubulin, beta 6 Calculated Molecular Weight: 50 kDa Observed Molecular Weight: 50 kDa GenBank Accession Number: NM_032525 Gene Symbol: Beta Tubulin Gene ID (NCBI): 84617 RRID: AB_2881742 Conjugate: Unconjugated Form: Liquid Purification Method: Protein G purification UNIPROT ID: Q9BUF5 Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20 o C storage. 20ul sizes contain 0.1% BSA.