Proteintech, 66368-1-Ig, NFIL3 Monoclonal antibody

Size: 20ul / 150ul The NFIL3 (66368-1-Ig) by Proteintech is a Monoclonal antibody targeting NFIL3 in WB, FC (Intra), ELISA applications with reactivity to human, pig samples 66368-1-Ig targets NFIL3 in WB, IF, FC (Intra), ELISA applications and shows reactivity with human, pig samples. Tested Applications Positive WB detected in: human skeletal muscle tissue Positive FC (Intra) detected in: NK92, NK92 cells Recommended dilution Western Blot (WB): WB : 1:500-1:2000 Flow Cytometry (FC) (INTRA): FC (INTRA) : 0.40 ug per 10^6 cells in a 100 µl suspension Background Information NFIL3, also named E4BP4 and IL3BP1, belongs to the bZIP family and NFIL3 subfamily. It represses transcription from promoters with activating transcription factor (ATF) sites. NFIL3 activates transcription from the interleukin-3 promoter in T-cells. It competes for the same consensus-binding site with PAR DNA-binding factors (DBP, HLF and TEF). It is a component of the circadian clock that acts as a negative regulator for the circadian expression of PER2 oscillation in the cell-autonomous core clock. NFIL3 protects pro-B cells from programmed cell death. It is a key regulator of TH2 responses(PMID:21499227). Specification Tested Reactivity: human, pig Cited Reactivity: human Host / Isotype: Mouse / IgG1 Class: Monoclonal Type: Antibody Immunogen: CatNo: Ag2380 Product name: Recombinant human NFIL3 protein Source: e coli. -derived, PGEX-4T Tag: GST Domain: 113-462 aa of BC008197 Sequence: ATLKAELLSLKLKFGLISSTAYAQEIQKLSNSTAVYFQDYQTSKSNVSSFVDEHEPSMVSSSCISVIKHSPQSSLSDVSEVSSVEHTQESSVQGSCRSPENKFQIIKQEPMELESYTREPRDDRGSYTASIYQNYMGNSFSGYSHSPPLLQVNRSSSNSPRTSETDDGVVGKSSDGEDEQQVPKGPIHSPVELKHVHATVVKVPEVNSSALPHKLRIKAKAMQIKVEAFDNEFEATQKLSSPIDMTSKRHFELEKHSAPSMVHSSLTPFSVQVTNIQDWSLKSEHWHQKELSGKTQNSFKTGVVEMKDSGYKVSDPENLYLKQGIANLSAEVVSLKRLIATQPISASDSG Predict reactive species Full Name: nuclear factor, interleukin 3 regulated Calculated Molecular Weight: 462 aa, 51 kDa Observed Molecular Weight: 50-55 kDa GenBank Accession Number: BC008197 Gene Symbol: NFIL3 Gene ID (NCBI): 4783 RRID: AB_2881748 Conjugate: Unconjugated Form: Liquid Purification Method: Protein G purification UNIPROT ID: Q16649 Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20 o C storage. 20ul sizes contain 0.1% BSA.