Proteintech, 66374-1-Ig, TINP1 Monoclonal antibody

Size: 20ul / 150ul The TINP1 (66374-1-Ig) by Proteintech is a Monoclonal antibody targeting TINP1 in WB, IHC, IF/ICC, ELISA applications with reactivity to human samples 66374-1-Ig targets TINP1 in WB, IHC, IF/ICC, ELISA applications and shows reactivity with human samples. Tested Applications Positive WB detected in: L02 cells, HEK-293 cells, HepG2 cells Positive IHC detected in: human breast cancer tissueNote: suggested antigen retrieval withTE buffer pH 9.0;(*) Alternatively, antigen retrieval may be performed withcitrate buffer pH 6.0 Positive IF/ICC detected in: HEK-293 cells Recommended dilution Western Blot (WB): WB : 1:1000-1:8000 Immunohistochemistry (IHC): IHC : 1:50-1:500 Immunofluorescence (IF)/ICC: IF/ICC : 1:200-1:800 Background Information TINP1 (TGF beta inducible nuclear protein 1) was widely expressed in various cell lines. TINP1 overexpression significantly promoted tumor cell proliferation, which may be associated with the downregulation of p53 expression. Furthermore, TINP1 promoted a number of cell lines to the S phase of the cell cycle. TINP1 promotes cell proliferation and significantly reduces p53 and p21 expression. Specification Tested Reactivity: human Host / Isotype: Mouse / IgG1 Class: Monoclonal Type: Antibody Immunogen: CatNo: Ag8822 Product name: Recombinant human TINP1 protein Source: e coli. -derived, PET28a Tag: 6*His Domain: 1-260 aa of BC005288 Sequence: MPQNEYIELHRKRYGYRLDYHEKKRKKESREAHERSKKAKKMIGLKAKLYHKQRHAEKIQMKKTIKMHEKRNTKQKNDEKTPQGAVPAYLLDREGQSRAKVLSNMIKQKRKEKAGKWEVPLPKVRAQGETEVLKVIRTGKRKKKAWKRMVTKVCFVGDGFTRKPPKYERFIRPMGLRFKKAHVTHPELKATFCLPILGVKKNPSSPLYTTLGVITKGTVIEVNVSELGLVTQGGKVIWGKYAQVTNNPENDGCINAVLLV Predict reactive species Full Name: TGF beta-inducible nuclear protein 1 Calculated Molecular Weight: 260 aa, 30 kDa Observed Molecular Weight: 34 kDa GenBank Accession Number: BC005288 Gene Symbol: TINP1 Gene ID (NCBI): 10412 RRID: AB_2881752 Conjugate: Unconjugated Form: Liquid Purification Method: Protein G purification UNIPROT ID: O95478 Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20 o C storage. 20ul sizes contain 0.1% BSA.