Proteintech, 66375-1-Ig, TUBB3-specific/TUJ1 Monoclonal antibody

Size: 20ul / 150ul The TUBB3-specific/TUJ1 (66375-1-Ig) by Proteintech is a Monoclonal antibody targeting TUBB3-specific/TUJ1 in WB, IHC, IF/ICC, IF-P, IF-Fro, FC (Intra), IP, ELISA applications with reactivity to human, mouse, rat, pig, rabbit, chicken samples 66375-1-Ig targets TUBB3-specific/TUJ1 in WB, IHC, IF/ICC, IF-P, IF-Fro, FC (Intra), IP, ELISA applications and shows reactivity with human, mouse, rat, pig, rabbit, chicken samples. Tested Applications Positive WB detected in: SH-SY5Y cells, fetal human brain tissue, HEK-293 cells, PC-12 cells, Neuro-2a cells, Pig brain, Rabbit brain, Rat brain Mouse brain, Chicken brain Positive IP detected in: SH-SY5Y cells Positive IHC detected in: human cerebellum tissue, mouse brain tissue, mouse cerebellum tissueNote: suggested antigen retrieval withTE buffer pH 9.0;(*) Alternatively, antigen retrieval may be performed withcitrate buffer pH 6.0 Positive IF-P detected in: rat brain tissue, Retinal organoids, mouse brain tissue, human neuron Positive IF-Fro detected in: rat brain tissue, rat cerebellum tissue Positive IF/ICC detected in: iPS cells Positive FC (Intra) detected in: SH-SY5Y cells Recommended dilution Western Blot (WB): WB : 1:5000-1:50000 Immunoprecipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate Immunohistochemistry (IHC): IHC : 1:400-1:20000 Immunofluorescence (IF)-P: IF-P : 1:50-1:500 Immunofluorescence (IF)-FRO: IF-FRO : 1:400-1:1600 Immunofluorescence (IF)/ICC: IF/ICC : 1:125-1:500 Flow Cytometry (FC) (INTRA): FC (INTRA) : 0.20 ug per 10^6 cells in a 100 µl suspension Background Information TUBB3, the class III β tubulin or Tuj1, is selectively expressed in testis and neurons of the central and peripheral nervous system. It has been widely used as a marker for neurons. Aberrant expression of TUBB3 has also been found in various tumors of non-neural origin and can be used as a biomarker for cancer aggressiveness and a marker for the tendency to respond poorly to chemotherapy. This antibody is specific to TUBB3 but not cross-react with other tubulin isoforms. Specification Tested Reactivity: human, mouse, rat, pig, rabbit, chicken Cited Reactivity: human, mouse, rat, chicken Host / Isotype: Mouse / IgG1 Class: Monoclonal Type: Antibody Immunogen: Peptide Predict reactive species Full Name: tubulin, beta 3 Calculated Molecular Weight: 55 kDa Observed Molecular Weight: 50-55 kDa GenBank Accession Number: NM_001197181 Gene Symbol: TUBB3 Gene ID (NCBI): 10381 RRID: AB_2814998 Conjugate: Unconjugated Form: Liquid Purification Method: Protein G purification UNIPROT ID: Q13509 Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20 o C storage. 20ul sizes contain 0.1% BSA.