Proteintech, 66376-1-Ig, Cardiac Troponin I Monoclonal antibody

Size: 20ul / 150ul The Cardiac Troponin I (66376-1-Ig) by Proteintech is a Monoclonal antibody targeting Cardiac Troponin I in WB, IHC, IF-P, ELISA applications with reactivity to human, mouse, rat, pig, rabbit samples 66376-1-Ig targets Cardiac Troponin I in WB, IHC, IF-P, ELISA, ISFET applications and shows reactivity with human, mouse, rat, pig, rabbit samples. Tested Applications Positive WB detected in: human heart tissue, rat heart tissue, mouse heart tissue, pig heart tissue, rabbit heart tissue Positive IHC detected in: human heart tissue, mouse heart tissueNote: suggested antigen retrieval withTE buffer pH 9.0;(*) Alternatively, antigen retrieval may be performed withcitrate buffer pH 6.0 Positive IF-P detected in: mouse heart tissue Recommended dilution Western Blot (WB): WB : 1:5000-1:50000 Immunohistochemistry (IHC): IHC : 1:50-1:500 Immunofluorescence (IF)-P: IF-P : 1:200-1:800 Background Information Troponin I (TnI) is the inhibitory subunit of troponin, the structural protein involved in the regulation of striated muscle contraction. Cardiac Troponin I (cTnI) is produced by cardiac muscle. Two other TnI isoforms (slow sTnI and fast sTnI) are produced by slow-twitch and fast-twitch skeletal muscles, respectively. cTnI has been considered as one of the most specific and sensitive markers of myocardial damage like acute myocardial infarction (AMI) for decades. Detection of cTnI in serum is widely accepted as a diagnostic tool in acute coronary syndromes. This antibody is specific to cTnI, but not cross-react with skeletal TnI. Specification Tested Reactivity: human, mouse, rat, pig, rabbit Cited Reactivity: human, mouse, rat, pig Host / Isotype: Mouse / IgG1 Class: Monoclonal Type: Antibody Immunogen: CatNo: Ag16758 Product name: Recombinant human TNNI3 protein Source: e coli. -derived, PET28a Tag: 6*His Domain: 1-110 aa of BC096165 Sequence: MADGSSDAAREPRPAPAPIRRRSSNYRAYATEPHAKKKSKISASRKLQLKTLLLQIAKQELEREAEERRGEKGRALSTRCQPLELAGLGFAELQDLCRQLHARVDKVDEE Predict reactive species Full Name: troponin I type 3 (cardiac) Calculated Molecular Weight: 210 aa, 24 kDa Observed Molecular Weight: 26-28 kDa GenBank Accession Number: BC096165 Gene Symbol: Cardiac Troponin I Gene ID (NCBI): 7137 RRID: AB_2881753 Conjugate: Unconjugated Form: Liquid Purification Method: Protein G purification UNIPROT ID: P19429 Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20 o C storage. 20ul sizes contain 0.1% BSA.