Proteintech, 66379-1-Ig, Beta Catenin Monoclonal antibody

Size: 20ul / 150ul The Beta Catenin (66379-1-Ig) by Proteintech is a Monoclonal antibody targeting Beta Catenin in WB, IHC, IF/ICC, IF-P, FC (Intra), IP, ELISA applications with reactivity to human, mouse, rat, pig samples 66379-1-Ig targets Beta Catenin in WB, IHC, IF/ICC, IF-P, FC (Intra), IP, CoIP, ELISA applications and shows reactivity with human, mouse, rat, pig samples. Tested Applications Positive WB detected in: A431 cells, pig brain tissue, rat brain tissue, mouse brain tissue, HeLa cells, HEK-293 cells, MCF-7 cells, LNCaP cells, HSC-T6 cells, NIH/3T3 cells, rabbit brain tissue Positive IP detected in: mouse brain tissue Positive IHC detected in: mouse colon tissue, human breast cancer tissue, human colon tissue, human ovary cancer tissue, human skin cancer tissue, rat colon tissueNote: suggested antigen retrieval withTE buffer pH 9.0;(*) Alternatively, antigen retrieval may be performed withcitrate buffer pH 6.0 Positive IF-P detected in: human colon cancer tissue, human skin cancer tissue Positive IF/ICC detected in: MCF-7 cells, human liver cancer tissue, human skin cancer tissue Positive FC (Intra) detected in: MCF-7 cells Recommended dilution Western Blot (WB): WB : 1:5000-1:50000 Immunoprecipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate Immunohistochemistry (IHC): IHC : 1:10000-1:40000 Immunofluorescence (IF)-P: IF-P : 1:200-1:800 Immunofluorescence (IF)/ICC: IF/ICC : 1:750-1:3000 Flow Cytometry (FC) (INTRA): FC (INTRA) : 0.50 ug per 10^6 cells in a 100 µl suspension Background Information β-Catenin, also known as CTNNB1, is an evolutionarily conserved, multifunctional intracellular protein. β-Catenin was originally identified in cell adherens junctions (AJs) where it functions to bridge the cytoplasmic domain of cadherins to a-catenin and the actin cytoskeleton. Besides its essential role in the AJs, β-catenin is also a key downstream component of the canonical Wnt pathway that plays diverse and critical roles in embryonic development and adult tissue homeostasis. The Wnt/β-catenin pathway is also involved in the activation of other intracellular messengers such as calcium fluxes, JNK, and SRC kinases. Deregulation of β-catenin activity is associated with multiple diseases including cancers. (PMID: 22617422; 18334222) Specification Tested Reactivity: human, mouse, rat, pig Cited Reactivity: human, mouse, rat, pig, chicken, sheep Host / Isotype: Mouse / IgG1 Class: Monoclonal Type: Antibody Immunogen: Peptide Predict reactive species Full Name: catenin (cadherin-associated protein), beta 1, 88kDa Calculated Molecular Weight: 781 aa, 86 kDa Observed Molecular Weight: 92 kDa GenBank Accession Number: NM_001904 Gene Symbol: Beta Catenin Gene ID (NCBI): 1499 ENSEMBL Gene ID: ENSG00000168036 RRID: AB_2857358 Conjugate: Unconjugated Form: Liquid Purification Method: Protein G purification UNIPROT ID: P35222 Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20 o C storage. 20ul sizes contain 0.1% BSA.