Proteintech, 66382-1-Ig, Osteoglycin Monoclonal antibody

Size: 20ul / 150ul The Osteoglycin (66382-1-Ig) by Proteintech is a Monoclonal antibody targeting Osteoglycin in WB, IHC, ELISA applications with reactivity to human, pig samples 66382-1-Ig targets Osteoglycin in WB, IHC, ELISA applications and shows reactivity with human, pig samples. Tested Applications Positive WB detected in: human artery tissue, pig artery tissue Positive IHC detected in: human liver tissue, human lung tissueNote: suggested antigen retrieval withTE buffer pH 9.0;(*) Alternatively, antigen retrieval may be performed withcitrate buffer pH 6.0 Recommended dilution Western Blot (WB): WB : 1:1000-1:6000 Immunohistochemistry (IHC): IHC : 1:50-1:500 Background Information This gene encodes a member of the small leucine-rich proteoglycan (SLRP) family of proteins. The encoded protein induces ectopic bone formation in conjunction with transforming growth factor beta and may regulate osteoblast differentiation. High expression of the encoded protein may be associated with elevated heart left ventricular mass. Alternative splicing results in multiple transcript variants which forms proteins with different molecular weight. Specification Tested Reactivity: human, pig Host / Isotype: Mouse / IgG1 Class: Monoclonal Type: Antibody Immunogen: CatNo: Ag3484 Product name: Recombinant human OGN protein Source: e coli. -derived, PGEX-4T Tag: GST Domain: 1-298 aa of BC037273 Sequence: MKTLQSTLLLLLLVPLIKPAPPTQQDSRIIYDYGTDNFEESIFSQDYEDKYLDGKNIKEKETVIIPNEKSLQLQKDEAITPLPPKKENDEMPTCLLCVCLSGSVYCEEVDIDAVPPLPKESAYLYARFNKIKKLTAKDFADIPNLRRLDFTGNLIEDIEDGTFSKLSLLEELSLAENQLLKLPVLPPKLTLFNAKYNKIKSRGIKANAFKKLNNLTFLYLDHNALESVPLNLPESLRVIHLQFNNIASITDDTFCKANDTSYIRDRIEEIRLEGNPIVLGKHPNSFICLKRLPIGSYF Predict reactive species Full Name: osteoglycin Calculated Molecular Weight: 298 aa, 34 kDa Observed Molecular Weight: 30-40 kDa GenBank Accession Number: BC037273 Gene Symbol: OGN Gene ID (NCBI): 4969 RRID: AB_2881758 Conjugate: Unconjugated Form: Liquid Purification Method: Protein G purification UNIPROT ID: P20774 Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20 o C storage. 20ul sizes contain 0.1% BSA.