Proteintech, 66484-1-Ig, human IgM Monoclonal antibody

Size: 20ul / 150ul The human IgM (66484-1-Ig) by Proteintech is a Monoclonal antibody targeting human IgM in WB, IHC, IF-P, ELISA applications with reactivity to human samples 66484-1-Ig targets human IgM in WB, IHC, IF-P, IP, ELISA applications and shows reactivity with human samples. Tested Applications Positive WB detected in: Human plasma tissue Positive IHC detected in: human tonsillitis tissue, human lymphoma tissueNote: suggested antigen retrieval withTE buffer pH 9.0;(*) Alternatively, antigen retrieval may be performed withcitrate buffer pH 6.0 Positive IF-P detected in: human tonsillitis tissue, human appendicitis tissue Recommended dilution Western Blot (WB): WB : 1:5000-1:20000 Immunohistochemistry (IHC): IHC : 1:2000-1:6000 Immunofluorescence (IF)-P: IF-P : 1:200-1:800 Background Information IGHM is the constant region of heavy chain of IgM. IgM is the first immunoglobulin expressed during B cell development. IgM antibodies play an important role in primary immune response involved in early recognition of external invaders like bacteria and viruses, cellular waste and modified self, as well as in recognition and elimination of precancerous and cancerous lesions. The μ heavy chain disease is a rare lymphoproliferative disorder. Human immunoglobulin heavy chain locus translocations are associated with leukaemias and lymphomas, including multiple myeloma, mantle cell lymphoma, Burkitt's lymphoma and diffuse large B cell lymphoma. This antibody detects the heavy chain of human IgM (~75 kDa). Specification Tested Reactivity: human Cited Reactivity: human Host / Isotype: Mouse / IgG1 Class: Monoclonal Type: Antibody Immunogen: CatNo: Ag1459 Product name: Recombinant human IGHM protein Source: e coli. -derived, PGEX-4T Tag: GST Domain: 1-351 aa of BC009851 Sequence: FGNPRKSKLICQATGFSPRQIQVSWLREGKQVGSGVTTDQVQAEAKESGPTTYKVTSTLTIKESDWLSQSMFTCRVDHRGLTFQQNASSMCVPDQDTAIRVFAIPPSFASIFLTKSTKLTCLVTDLTTYDSVTISWTRQNGEAVKTHTNISESHPNATFSAVGEASICEDDWNSGERFTCTVTHTDLPSPLKQTISRPKGVALHRPDVYLLPPAREQLNLRESATITCLVTGFSPADVFVQWMQRGQPLSPEKYVTSAPMPEPQAPGRYFAHSILTVSEEEWNTGETYTCVVAHEALPNRVTERTVDKSTEGEVSADEEGFENLWATASTFIVLFLLSLFYSTTVTLFKVK Predict reactive species Full Name: immunoglobulin heavy constant mu Calculated Molecular Weight: 69 kDa Observed Molecular Weight: 75 kDa GenBank Accession Number: BC009851 Gene Symbol: IGHM Gene ID (NCBI): 3507 RRID: AB_2881850 Conjugate: Unconjugated Form: Liquid Purification Method: Protein G purification Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20 o C storage. 20ul sizes contain 0.1% BSA.