Proteintech, 66516-1-Ig, SMAD3 Monoclonal antibody

Size: 20ul / 150ul The SMAD3 (66516-1-Ig) by Proteintech is a Monoclonal antibody targeting SMAD3 in WB, IHC, ELISA applications with reactivity to human, mouse samples 66516-1-Ig targets SMAD3 in WB, IHC, IF, IP, CoIP, ChIP, ELISA applications and shows reactivity with human, mouse samples. Tested Applications Positive WB detected in: HT-1080 cells, NIH/3T3 cells, A431 cells, HEK-293 cells, Jurkat cells, K-562 cells, 4T1 cells Positive IHC detected in: human stomach cancer tissue, mouse lung tissueNote: suggested antigen retrieval withTE buffer pH 9.0;(*) Alternatively, antigen retrieval may be performed withcitrate buffer pH 6.0 Recommended dilution Western Blot (WB): WB : 1:2000-1:12000 Immunohistochemistry (IHC): IHC : 1:500-1:2000 Background Information SMAD3, also named as hMAD 3 or Mad3, is a 425 amino acid protein, which contains one MH1 domain and one MH2 domain. SMAD3 localizes in the nucleus and cytoplasm. SMAD3 plays an essential role in development and maintenance of self-tolerance and is a critical mediator of the TGFB signaling pathway. SMAD3 is involved in TGFB dependent regulation of steroidogenesis and in T-cell response to TGFB. Specification Tested Reactivity: human, mouse Cited Reactivity: human, mouse, pig, rabbit Host / Isotype: Mouse / IgG1 Class: Monoclonal Type: Antibody Immunogen: Peptide Predict reactive species Full Name: SMAD family member 3 Calculated Molecular Weight: 48 kDa Observed Molecular Weight: 55-60 kDa GenBank Accession Number: NM_005902 Gene Symbol: SMAD3 Gene ID (NCBI): 4088 RRID: AB_2881879 Conjugate: Unconjugated Form: Liquid Purification Method: Protein G purification UNIPROT ID: P84022 Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20 o C storage. 20ul sizes contain 0.1% BSA.