Proteintech, 66549-1-Ig, HuR/ELAVL1 Monoclonal antibody

Size: 20ul / 150ul The HuR/ELAVL1 (66549-1-Ig) by Proteintech is a Monoclonal antibody targeting HuR/ELAVL1 in WB, IHC, IF/ICC, ELISA applications with reactivity to Human, mouse, rat samples 66549-1-Ig targets HuR/ELAVL1 in WB, IHC, IF/ICC, ELISA applications and shows reactivity with Human, mouse, rat samples. Tested Applications Positive WB detected in: HeLa cells, HEK-293 cells, A549 cells, MCF-7 cells, Jurkat cells, HSC-T6 cells, PC-12 cells, NIH/3T3 cells, Neuro-2a cells, RAW 264.7 cells, NCI-H1299 cells Positive IHC detected in: human ovary tumor tissue, human breast cancer tissueNote: suggested antigen retrieval withTE buffer pH 9.0;(*) Alternatively, antigen retrieval may be performed withcitrate buffer pH 6.0 Positive IF/ICC detected in: HepG2 cells Recommended dilution Western Blot (WB): WB : 1:5000-1:50000 Immunohistochemistry (IHC): IHC : 1:500-1:2000 Immunofluorescence (IF)/ICC: IF/ICC : 1:200-1:800 Background Information ELAVL1, also named as HUR, belongs to the RRM elav family. It is involved in 3'-UTR ARE-mediated MYC stabilization. ELAVL1 binds avidly to the AU-rich element in FOS and IL3/interleukin-3 mRNAs. In the case of the FOS AU-rich element, ELAVL1 binds to a core element of 27 nucleotides that contain AUUUA, AUUUUA and AUUUUUA motifs. Specification Tested Reactivity: Human, mouse, rat Cited Reactivity: human, mouse Host / Isotype: Mouse / IgG2a Class: Monoclonal Type: Antibody Immunogen: CatNo: Ag2249 Product name: Recombinant human HuR protein Source: e coli. -derived, PGEX-4T Tag: GST Domain: 1-326 aa of BC003376 Sequence: MSNGYEDHMAEDCRGDIGRTNLIVNYLPQNMTQDELRSLFSSIGEVESAKLIRDKVAGHSLGYGFVNYVTAKDAERAINTLNGLRLQSKTIKVSYARPSSEVIKDANLYISGLPRTMTQKDVEDMFSRFGRIINSRVLVDQTTGLSRGVAFIRFDKRSEAEEAITSFNGHKPPGSSEPITVKFAANPNQNKNVALLSQLYHSPARRFGGPVHHQAQRFRFSPMGVDHMSGLSGVNVPGNASSGWCIFIYNLGQDADEGILWQMFGPFGAVTNVKVIRDFNTNKCKGFGFVTMTNYEEAAMAIASLNGYRLGDKILQVSFKTNKSHK Predict reactive species Full Name: ELAV (embryonic lethal, abnormal vision, Drosophila)-like 1 (Hu antigen R) Calculated Molecular Weight: 326 aa, 36 kDa Observed Molecular Weight: 34-37 kDa GenBank Accession Number: BC003376 Gene Symbol: HuR/ELAVL1 Gene ID (NCBI): 1994 RRID: AB_2881911 Conjugate: Unconjugated Form: Liquid Purification Method: Protein A purification UNIPROT ID: Q15717 Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20 o C storage. 20ul sizes contain 0.1% BSA.