Proteintech, 66571-1-Ig, CIN85 Monoclonal antibody

Size: 20ul / 150ul The CIN85 (66571-1-Ig) by Proteintech is a Monoclonal antibody targeting CIN85 in WB, ELISA applications with reactivity to Human, mouse, rat samples 66571-1-Ig targets CIN85 in WB, ELISA applications and shows reactivity with Human, mouse, rat samples. Tested Applications Positive WB detected in: HL-60 cells, rat brain tissue, K-562 cells, PC-12 cells, mouse brain tissue, THP-1 cells, Jurkat cells, Ramos cells, Raji cells, Daudi cells Recommended dilution Western Blot (WB): WB : 1:2000-1:10000 Background Information CIN85 is identified as a Cbl-interacting protein of 85 kDa and belongs to adaptor/scaffold proteins. It composed of three N-terminal SH3 domains followed by a proline-rich region and a C-terminal coiled-coil region. In association with c-Cbl (Casitas B-lineage lymphoma), an E3 ubiquitin ligase. As an adaptor protein, CIN85 is involved in Cbl-dependent EGFR internalization, intracellular receptor trafficking, sorting and degradation. Moreover, through the SH3 domains and the proline-rich region, CIN85 is implicated in many protein-protein interactions and it is found to play important roles in other processes such as apoptosis, rearrangement of actin cytoskeleton, cell adhesion, immunological synapse, cell migration and invasion. Specification Tested Reactivity: Human, mouse, rat Host / Isotype: Mouse / IgG1 Class: Monoclonal Type: Antibody Immunogen: CatNo: Ag2777 Product name: Recombinant human CIN85 protein Source: e coli. -derived, PGEX-4T Tag: GST Domain: 1-328 aa of BC015806 Sequence: MVEAIVEFDYQAQHDDELTISVGEIITNIRKEDGGWWEGQINGRRGLFPDNFVREIKKEMKKDPLTNKAPEKPLHEVPSGNSLLSSETILRTNKRGERRRRRCQVAFSYLPQNDDELELKVGDIIEVVGEVEEGWWEGVLNGKTGMFPSNFIKELSGESDELGISQDEQLSKSSLRETTGSESDGGDSSSTKSEGANGTVATAAIQPKKVKGVGFGDIFKDKPIKLRPRSIEVENDFLPVEKTIGKKLPATTATPDSSKTEMDSRTKSKDYCKVIFPYEAQNDDELTIKEGDIVTLINKDCIDVGWWEGELNGRRGVFPDNFVKLLPP Predict reactive species Full Name: SH3-domain kinase binding protein 1 Calculated Molecular Weight: 665 aa, 73 kDa Observed Molecular Weight: 85 kDa GenBank Accession Number: BC015806 Gene Symbol: SH3KBP1/CIN85 Gene ID (NCBI): 30011 RRID: AB_2881932 Conjugate: Unconjugated Form: Liquid Purification Method: Protein G purification UNIPROT ID: Q96B97 Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20 o C storage. 20ul sizes contain 0.1% BSA.