Proteintech, 66574-1-Ig, GRP78/BIP Monoclonal antibody

Size: 20ul / 150ul The GRP78/BIP (66574-1-Ig) by Proteintech is a Monoclonal antibody targeting GRP78/BIP in WB, IHC, IF/ICC, IP, ELISA applications with reactivity to human, mouse, rat samples 66574-1-Ig targets GRP78/BIP in WB, IHC, IF/ICC, IP, CoIP, ELISA applications and shows reactivity with human, mouse, rat samples. Tested Applications Positive WB detected in: HepG2 cells, HeLa cells, A549 cells, MCF-7 cells, Jurkat cells, HSCT6 cells, HSC-T6 cells, PC-12 cells, NIH/3T3 cells, SP2/0 cell, 4T1 cells Positive IP detected in: SGC-7901 cells Positive IHC detected in: human liver cancer tissue, human colon cancer tissue, human breast cancer tissueNote: suggested antigen retrieval withTE buffer pH 9.0;(*) Alternatively, antigen retrieval may be performed withcitrate buffer pH 6.0 Positive IF/ICC detected in: thapsigargin treated U2OS cells Recommended dilution Western Blot (WB): WB : 1:5000-1:50000 Immunoprecipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate Immunohistochemistry (IHC): IHC : 1:500-1:2000 Immunofluorescence (IF)/ICC: IF/ICC : 1:400-1:1600 Background Information GRP78 (HSPA5), also referred to as 'immunoglobulin heavy chain-binding protein' (BiP), is a member of the heat-shock protein-70 (HSP70) family and is involved in the folding and assembly of proteins in the endoplasmic reticulum (ER).It is a constitutively expressed resident protein of the ER in all eukaryotic cells. Recently it has been reported that GRP78 is associated with apoptosis or inhibition of cancer cell growth. Specification Tested Reactivity: human, mouse, rat Cited Reactivity: human, mouse, rat, pig, chicken, zebrafish Host / Isotype: Mouse / IgG1 Class: Monoclonal Type: Antibody Immunogen: CatNo: Ag2188 Product name: Recombinant human GRP78,BIP protein Source: e coli. -derived, PGEX-4T Tag: GST Domain: 351-654 aa of BC020235 Sequence: LKKSDIDEIVLVGGSTRIPKIQQLVKEFFNGKEPSRGINPDEAVAYGAAVQAGVLSGDQDTGDLVLLDVCPLTLGIETVGGVMTKLIPRNTVVPTKKSQIFSTASDNQPTVTIKVYEGERPLTKDNHLLGTFDLTGIPPAPRGVPQIEVTFEIDVNGILRVTAEDKGTGNKNKITITNDQNRLTPEEIERMVNDAEKFAEEDKKLKERIDTRNELESYAYSLKNQIGDKEKLGGKLSSEDKETMEKAVEEKIEWLESHQDADIEDFKAKKKELEEIVQPIISKLYGSAGPPPTGEEDTAEKDEL Predict reactive species Full Name: heat shock 70kDa protein 5 (glucose-regulated protein, 78kDa) Calculated Molecular Weight: 78 kDa Observed Molecular Weight: 70-78 kDa GenBank Accession Number: BC020235 Gene Symbol: GRP78/BIP Gene ID (NCBI): 3309 RRID: AB_2881934 Conjugate: Unconjugated Form: Liquid Purification Method: Protein G purification UNIPROT ID: P11021 Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20 o C storage. 20ul sizes contain 0.1% BSA.