Proteintech, 66576-1-Ig, c-Cbl Monoclonal antibody

Size: 20ul / 150ul The c-Cbl (66576-1-Ig) by Proteintech is a Monoclonal antibody targeting c-Cbl in WB, IHC, ELISA applications with reactivity to Human samples 66576-1-Ig targets c-Cbl in WB, IHC, ELISA applications and shows reactivity with Human samples. Tested Applications Positive WB detected in: HL-60 cells, Daudi cells, K-562 cells, Jurkat cells, THP-1 cells, Raji cells Positive IHC detected in: human tonsillitis tissueNote: suggested antigen retrieval withTE buffer pH 9.0;(*) Alternatively, antigen retrieval may be performed withcitrate buffer pH 6.0 Recommended dilution Western Blot (WB): WB : 1:5000-1:50000 Immunohistochemistry (IHC): IHC : 1:300-1:1200 Background Information c-Cbl (Casitas B-lineage lymphoma) is a critical regulator that controls immature or follicular helper T cell development, B cell development, immune tolerance, angiogenesis, or tumorigenesis (PMID: 33962939). c-Cbl also plays a pivotal role in regulating dendritic cell (DC) function via negatively modulating IL-12 production after Toll-like receptor (TLR) stimulation (PMID: 21799517). Specification Tested Reactivity: Human Cited Reactivity: human Host / Isotype: Mouse / IgG1 Class: Monoclonal Type: Antibody Immunogen: CatNo: Ag23033 Product name: Recombinant human CBL protein Source: e coli. -derived, PGEX-4T Tag: GST Domain: 578-906 aa of BC132733 Sequence: LPPVPSSRLGDSWLPRPIPKVPVSAPSSSDPWTGRELTNRHSLPFSLPSQMEPRPDVPRLGSTFSLDTSMSMNSSPLVGPECDHPKIKPSSSANAIYSLAARPLPVPKLPPGEQCEGEEDTEYMTPSSRPLRPLDTSQSSRACDCDQQIDSCTYEAMYNIQSQAPSITESSTFGEGNLAAAHANTGPEESENEDDGYDVPKPPVPAVLARRTLSDISNASSSFGWLSLDGDPTTNVTEGSQVPERPPKPFPRRINSERKAGSCQQGSGPAASAATASPQLSSEIENLMSQGYSYQDIQKALVIAQNNIEMAKNILREFVSISSPAHVAT Predict reactive species Full Name: Cas-Br-M (murine) ecotropic retroviral transforming sequence Calculated Molecular Weight: 906 aa, 100 kDa Observed Molecular Weight: 100-120 kDa GenBank Accession Number: BC132733 Gene Symbol: CBL Gene ID (NCBI): 867 RRID: AB_2881936 Conjugate: Unconjugated Form: Liquid Purification Method: Protein A purification UNIPROT ID: P22681 Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20 o C storage. 20ul sizes contain 0.1% BSA.