Proteintech, 66587-1-Ig, DNAJB6 Monoclonal antibody

Size: 20ul / 150ul The DNAJB6 (66587-1-Ig) by Proteintech is a Monoclonal antibody targeting DNAJB6 in WB, IHC, IF/ICC, ELISA applications with reactivity to human, mouse, rat, pig samples 66587-1-Ig targets DNAJB6 in WB, IHC, IF/ICC, ELISA applications and shows reactivity with human, mouse, rat, pig samples. Tested Applications Positive WB detected in: Jurkat cells, HEK-293 cells, HSC-T6 cells, Neuro-2a cells, pig brain tissue, rat brain tissue, mouse brain tissue, HeLa cells Positive IHC detected in: human testis tissue, mouse brain tissueNote: suggested antigen retrieval withTE buffer pH 9.0;(*) Alternatively, antigen retrieval may be performed withcitrate buffer pH 6.0 Positive IF/ICC detected in: HepG2 cells Recommended dilution Western Blot (WB): WB : 1:2000-1:8000 Immunohistochemistry (IHC): IHC : 1:500-1:2000 Immunofluorescence (IF)/ICC: IF/ICC : 1:200-1:800 Background Information DNAJB6, belongs to the heat shock protein 40 (HSP40) family, a noncanonical member of the DNAJ-chaperone family, is mainly expressed in neurons. It plays an important role in recovery from misfolded protein aggregates (PMID: 27276715). As seen by the western blot stained for DNAJB6, there are 2 splice forms of the protein resulting in a longer isoform (38 kDa) and a shorter one (27 kDa). DNAJB6 (27 kDa) isoform but not DNAJB6 (38 kDa) isoform is effective with suppressing cytoplasmic protein aggregation (PMID: 20159555). The antibody 66587-1-Ig recognizes both isoforms. Specification Tested Reactivity: human, mouse, rat, pig Cited Reactivity: human Host / Isotype: Mouse / IgG1 Class: Monoclonal Type: Antibody Immunogen: CatNo: Ag2258 Product name: Recombinant human DNAJB6 protein Source: e coli. -derived, PGEX-4T Tag: GST Domain: 1-241 aa of BC000177 Sequence: MVDYYEVLGVQRHASPEDIKKAYRKLALKWHPDKNPENKEEAERKFKQVAEAYEVLSDAKKRDIYDKYGKEGLNGGGGGGSHFDSPFEFGFTFRNPDDVFREFFGGRDPFSFDFFEDPFEDFFGNRRGPRGSRSRGTGSFFSAFSGFPSFGSGFSSFDTGFTSFGSLGHGGLTSFSSTSFGGSGMGNFKSISTSTKMVNGRKITTKRIVENGQERVEVEEDGQLKSLTINGKEQLLRLDNK Predict reactive species Full Name: DnaJ (Hsp40) homolog, subfamily B, member 6 Calculated Molecular Weight: 241 aa, 27 kDa Observed Molecular Weight: 27 kDa, 38 kDa GenBank Accession Number: BC000177 Gene Symbol: DNAJB6 Gene ID (NCBI): 10049 RRID: AB_2881947 Conjugate: Unconjugated Form: Liquid Purification Method: Protein G purification UNIPROT ID: O75190 Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20 o C storage. 20ul sizes contain 0.1% BSA.