Proteintech, 66596-1-Ig, ACAP1 Monoclonal antibody

Size: 20ul / 150ul The ACAP1 (66596-1-Ig) by Proteintech is a Monoclonal antibody targeting ACAP1 in WB, IHC, ELISA applications with reactivity to human samples 66596-1-Ig targets ACAP1 in WB, IHC, ELISA applications and shows reactivity with human samples. Tested Applications Positive WB detected in: Jurkat cells, HL-60 cells, K-562 cells Positive IHC detected in: human breast cancer tissueNote: suggested antigen retrieval withTE buffer pH 9.0;(*) Alternatively, antigen retrieval may be performed withcitrate buffer pH 6.0 Recommended dilution Western Blot (WB): WB : 1:1000-1:6000 Immunohistochemistry (IHC): IHC : 1:150-1:600 Background Information ACAP1, also named as Centaurin-beta-1, is a 740 amino acid protein, which is expressed Highly in lung and spleen. ACAP1 as a GTPase-activating protein (GAP) for ADP ribosylation factor 6 (ARF6) is required for clathrin-dependent export of proteins from recycling endosomes to trans-Golgi network and cell surface and required for regulated export of ITGB1 from recycling endosomes to the cell surface and ITGB1-dependent cell migration. Specification Tested Reactivity: human Cited Reactivity: human Host / Isotype: Mouse / IgG1 Class: Monoclonal Type: Antibody Immunogen: CatNo: Ag14254 Product name: Recombinant human ACAP1 protein Source: e coli. -derived, PET28a Tag: 6*His Domain: 20-251 aa of BC018543 Sequence: SIELVEAEVSELETRLEKLLKLGTGLLESGRHYLAASRAFVVGICDLARLGPPEPMMAECLEKFTVSLNHKLDSHAELLDATQHTLQQQIQTLVKEGLRGFREARRDFWRGAESLEAALTHNAEVPRRRAQEAEEAGAALRTARAGYRGRALDYALQINVIEDKRKFDIMEFVLRLVEAQATHFQQGHEELSRLSQYRKELGAQLHQLVLNSAREKRDMEQRHVLLKQKELG Predict reactive species Full Name: ArfGAP with coiled-coil, ankyrin repeat and PH domains 1 Calculated Molecular Weight: 740 aa, 82 kDa Observed Molecular Weight: 81 kDa GenBank Accession Number: BC018543 Gene Symbol: ACAP1 Gene ID (NCBI): 9744 RRID: AB_2881956 Conjugate: Unconjugated Form: Liquid Purification Method: Protein G purification UNIPROT ID: Q15027 Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20 o C storage. 20ul sizes contain 0.1% BSA.