Proteintech, 66628-1-Ig, ACTN4 Monoclonal antibody

Size: 20ul / 150ul The ACTN4 (66628-1-Ig) by Proteintech is a Monoclonal antibody targeting ACTN4 in WB, IHC, ELISA applications with reactivity to human, mouse, rat samples 66628-1-Ig targets ACTN4 in WB, IHC, IF, IP, ELISA applications and shows reactivity with human, mouse, rat samples. Tested Applications Positive WB detected in: HeLa cells, LNCaP cells, HEK-293 cells, MCF-7 cells, Jurkat cells, HSC-T6 cells, NIH/3T3 cells, 4T1 cells, K-562 cells, pig brain tissue, mouse brain tissue, rat brain tissue, chicken brain tissue Positive IHC detected in: human breast cancer tissue, human lung cancer tissueNote: suggested antigen retrieval withTE buffer pH 9.0;(*) Alternatively, antigen retrieval may be performed withcitrate buffer pH 6.0 Recommended dilution Western Blot (WB): WB : 1:5000-1:20000 Immunohistochemistry (IHC): IHC : 1:2500-1:10000 Background Information ACTN4 (alpha actinin 4) is a ubiquitous actin-binding protein that cross-links actin filaments into bundles to form filopodia, and has a crucial role in cytoskeletal organization. ACTN4 is colocalized with actin stress fibers and is dispersed in the cytoplasm and in the nucleus. Increased levels of ACTN4 have been associated with cancer metastasis, indicating its implication in cancer cell migration and metastasis. Defects in ACTN4 cause glomerular disease. This antibody recognizes 105 and 80 kDa of endogenous ACTN4 protein in various lysates. (22370640, 10700177) Specification Tested Reactivity: human, mouse, rat Cited Reactivity: human Host / Isotype: Mouse / IgG1 Class: Monoclonal Type: Antibody Immunogen: CatNo: Ag13549 Product name: Recombinant human ACTN4 protein Source: e coli. -derived, PET28a Tag: 6*His Domain: 612-911 aa of BC005033 Sequence: TTVTPQIINSKWEKVQQLVPKRDHALLEEQSKQQSNEHLRRQFASQANVVGPWIQTKMEEIGRISIEMNGTLEDQLSHLKQYERSIVDYKPNLDLLEQQHQLIQEALIFDNKHTNYTMEHIRVGWEQLLTTIARTINEVENQILTRDAKGISQEQMQEFRASFNHFDKDHGGALGPEEFKACLISLGYDVENDRQGEAEFNRIMSLVDPNHSGLVTFQAFIDFMSRETTDTDTADQVIASFKVLAGDKNFITAEELRRELPPDQAEYCIARMAPYQGPDAVPGALDYKSFSTALYGESDL Predict reactive species Full Name: actinin, alpha 4 Calculated Molecular Weight: 105 kDa Observed Molecular Weight: 105 and 80 kDa GenBank Accession Number: BC005033 Gene Symbol: ACTN4 Gene ID (NCBI): 81 RRID: AB_2881988 Conjugate: Unconjugated Form: Liquid Purification Method: Protein G purification UNIPROT ID: O43707 Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20 o C storage. 20ul sizes contain 0.1% BSA.