Proteintech, 66645-1-Ig, TLR2 Monoclonal antibody

Size: 20ul / 150ul The TLR2 (66645-1-Ig) by Proteintech is a Monoclonal antibody targeting TLR2 in WB, IHC, IF/ICC, ELISA applications with reactivity to human samples 66645-1-Ig targets TLR2 in WB, IHC, IF/ICC, ELISA applications and shows reactivity with human samples. Tested Applications Positive WB detected in: THP-1 cells, U-937 cells Positive IHC detected in: human liver tissueNote: suggested antigen retrieval withTE buffer pH 9.0;(*) Alternatively, antigen retrieval may be performed withcitrate buffer pH 6.0 Positive IF/ICC detected in: THP-1 cells Recommended dilution Western Blot (WB): WB : 1:500-1:2000 Immunohistochemistry (IHC): IHC : 1:200-1:800 Immunofluorescence (IF)/ICC: IF/ICC : 1:50-1:500 Background Information TLR2, also known as CD282, is a Toll-like receptor (TLR) family member. TLR receptors are type I integral transmembrane glycoproteins that play a fundamental role in pathogen recognition and activation of innate immunity. TLR2 is expressed by immune cells including monocytes/macrophages, polymorphonuclear leukocytes (PMLs), and dendritic cells, and is also expressed by some no-immune cells including endothelial and epithelial cells (PMID: 21428911; 22566960). TLR2 forms heterodimers with TLR1 and TLR6, which is the initial step in a cascade of events leading to significant innate immune responses, development of adaptive immunity to pathogens, and protection from immune sequelae related to infection with these pathogens (PMID: 22566960). Specification Tested Reactivity: human Cited Reactivity: human, mouse Host / Isotype: Mouse / IgG2a Class: Monoclonal Type: Antibody Immunogen: CatNo: Ag25626 Product name: Recombinant human TLR2 protein Source: e coli. -derived, PET28a Tag: 6*His Domain: 19-302 aa of BC033756 Sequence: KEESSNQASLSCDRNGICKGSSGSLNSIPSGLTEAVKSLDLSNNRITYISNSDLQRCVNLQALVLTSNGINTIEEDSFSSLGSLEHLDLSYNYLSNLSSSWFKPLSSLTFLNLLGNPYKTLGETSLFSHLTKLQILRVGNMDTFTKIQRKDFAGLTFLEELEIDASDLQSYEPKSLKSIQNVSHLILHMKQHILLLEIFVDVTSSVECLELRDTDLDTFHFSELSTGETNSLIKKFTFRNVKITDESLFQVMKLLNQISGLLELEFDDCTLNGVGNFRASDNDR Predict reactive species Full Name: toll-like receptor 2 Calculated Molecular Weight: 784 aa, 90 kDa Observed Molecular Weight: 90-100 kDa GenBank Accession Number: BC033756 Gene Symbol: TLR2 Gene ID (NCBI): 7097 RRID: AB_2882003 Conjugate: Unconjugated Form: Liquid Purification Method: Protein A purification UNIPROT ID: O60603 Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20 o C storage. 20ul sizes contain 0.1% BSA.