Proteintech, 66679-1-Ig, BBS10 Monoclonal antibody

Size: 20ul / 150ul The BBS10 (66679-1-Ig) by Proteintech is a Monoclonal antibody targeting BBS10 in WB, IHC, ELISA applications with reactivity to Human samples 66679-1-Ig targets BBS10 in WB, IHC, ELISA applications and shows reactivity with Human samples. Tested Applications Positive WB detected in: NCI-H1299 cells, NCCIT cells, L02 cells, HepG2 cells Positive IHC detected in: human liver cancer tissueNote: suggested antigen retrieval withTE buffer pH 9.0;(*) Alternatively, antigen retrieval may be performed withcitrate buffer pH 6.0 Recommended dilution Western Blot (WB): WB : 1:1000-1:6000 Immunohistochemistry (IHC): IHC : 1:150-1:600 Background Information BBS10, also named as C12orf58, belongs to the TCP-1 chaperonin family. It is probable molecular chaperone. Assists the folding of proteins upon ATP hydrolysis. As part of the BBS/CCT complex, BBS10 may play a role in the assembly of BBSome, a complex involved in ciliogenesis regulating transports vesicles to the cilia. Involved in adipogenic differentiation. (PMID: 20080638, 19190184) Defects in BBS10 are the cause of Bardet-Biedl syndrome type 10 (BBS10). Specification Tested Reactivity: Human Host / Isotype: Mouse / IgG2b Class: Monoclonal Type: Antibody Immunogen: CatNo: Ag17960 Product name: Recombinant human BBS10 protein Source: e coli. -derived, PET28a Tag: 6*His Domain: 573-723 aa of BC013795 Sequence: MLPVSCKLPNMGTSQSYLSSSMPAGCVLPVGGNFEILLHYYLLNYAKKCHQSEETMVSMIIANALLGIPKVLYKSKTGKYSFPHTYIRAVHALQTNQPLVSSQTGLESVMGKYQLLTSVLQCLTKILTIDMVITVKRHPQKVHNQDSEDEL Predict reactive species Full Name: Bardet-Biedl syndrome 10 Calculated Molecular Weight: 81 kDa Observed Molecular Weight: 81 kDa GenBank Accession Number: BC013795 Gene Symbol: BBS10 Gene ID (NCBI): 79738 RRID: AB_2882033 Conjugate: Unconjugated Form: Liquid Purification Method: Protein A purification UNIPROT ID: Q8TAM1 Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20 o C storage. 20ul sizes contain 0.1% BSA.