Proteintech, 66702-1-Ig, TRIB3 Monoclonal antibody

Size: 20ul / 150ul The TRIB3 (66702-1-Ig) by Proteintech is a Monoclonal antibody targeting TRIB3 in WB, ELISA applications with reactivity to human, mouse, rat samples 66702-1-Ig targets TRIB3 in WB, ELISA applications and shows reactivity with human, mouse, rat samples. Tested Applications Positive WB detected in: HT-29 cells, MCF-7 cells, rat liver tissue, mouse hepatocytes, DU 145 cells, LNCaP cells, PC-3 cells, HUVEC cells, HepG2 cells, L02 cells Recommended dilution Western Blot (WB): WB : 1:2000-1:10000 Background Information TRIB3 is a pseudokinase molecule that affects a number of cellular functions. TRIB3 has been reported to be highly activated in the presence of a variety of stressors, including the deprivation of neurotrophic factors, hypoxia, and ER stress.TRIB3 protein is associated with a good prognosis in human breast cancer patients, possibly due to the fact that TRIB3 is involved in hypoxia tolerance.(PMID: 21704407). TRIB3 protein predicted molecular weights is approximately 39~45 kDa.(PMID: 31191318, PMID: 24414038, PMID: 31578236). Specification Tested Reactivity: human, mouse, rat Cited Reactivity: human, mouse Host / Isotype: Mouse / IgG1 Class: Monoclonal Type: Antibody Immunogen: CatNo: Ag25776 Product name: Recombinant human TRIB3 protein Source: e coli. -derived, PET28a Tag: 6*His Domain: 1-358 aa of BC027484 Sequence: MRATPLAAPAGSLSRKKRLELDDNLDTERPVQKRARSGPQPRLPPCLLPLSPPTAPDRATAVATASRLGPYVLLEPEEGGRAYRALHCPTGTEYTCKVYPVQEALAVLEPYARLPPHKHVARPTEVLAGTQLLYAFFTRTHGDMHSLVRSRHRIPEPEAAVLFRQMATALAHCHQHGLVLRDLKLCRFVFADRERKKLVLENLEDSCVLTGPDDSLWDKHACPAYVGPEILSSRASYSGKAADVWSLGVALFTMLAGHYPFQDSEPVLLFGKIRRGAYALPAGLSAPARCLVRCLLRREPAERLTATGILLHPWLRQDPMPLAPTRSHLWEAAQVVPDGLGLDEAREEEGDREVVLYG Predict reactive species Full Name: tribbles homolog 3 (Drosophila) Calculated Molecular Weight: 358 aa, 40 kDa Observed Molecular Weight: 40-45 kDa GenBank Accession Number: BC027484 Gene Symbol: TRIB3 Gene ID (NCBI): 57761 RRID: AB_2882055 Conjugate: Unconjugated Form: Liquid Purification Method: Protein G purification UNIPROT ID: Q96RU7 Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20 o C storage. 20ul sizes contain 0.1% BSA.