Proteintech, 66713-1-Ig, TPT1 Monoclonal antibody

Size: 20ul / 150ul The TPT1 (66713-1-Ig) by Proteintech is a Monoclonal antibody targeting TPT1 in WB, IHC, IF/ICC, ELISA applications with reactivity to human, mouse, rat samples 66713-1-Ig targets TPT1 in WB, IHC, IF/ICC, ELISA applications and shows reactivity with human, mouse, rat samples. Tested Applications Positive WB detected in: Jurkat cells, HeLa cells, RAW 264.7 cells, MCF-7 cells, HSC-T6 cells, NIH/3T3 cells Positive IHC detected in: human colon cancer tissueNote: suggested antigen retrieval withTE buffer pH 9.0;(*) Alternatively, antigen retrieval may be performed withcitrate buffer pH 6.0 Positive IF/ICC detected in: HEK-293 cells Recommended dilution Western Blot (WB): WB : 1:5000-1:50000 Immunohistochemistry (IHC): IHC : 1:500-1:2000 Immunofluorescence (IF)/ICC: IF/ICC : 1:200-1:800 Background Information The translationally controlled tumor protein (TPT1, also known as TCTP) is a highly conserved, abundantly expressed protein associated with cell growth and apoptosis regulation. It is overexpressed in most tumor cells and its down-regulation decreases the viability of those cells. This antibody detected 22 kDa TPT1 in lysates. Specification Tested Reactivity: human, mouse, rat Host / Isotype: Mouse / IgG1 Class: Monoclonal Type: Antibody Immunogen: CatNo: Ag27651 Product name: Recombinant human TPT1 protein Source: e coli. -derived, PET28a Tag: 6*His Domain: 1-172 aa of BC003352 Sequence: MIIYRDLISHDEMFSDIYKIREIADGLCLEVEGKMVSRTEGNIDDSLIGGNASAEGPEGEGTESTVITGVDIVMNHHLQETSFTKEAYKKYIKDYMKSIKGKLEEQRPERVKPFMTGAAEQIKHILANFKNYQFFIGENMNPDGMVALLDYREDGVTPYMIFFKDGLEMEKC Predict reactive species Full Name: tumor protein, translationally-controlled 1 Calculated Molecular Weight: 20 kDa Observed Molecular Weight: 22 kDa GenBank Accession Number: BC003352 Gene Symbol: TPT1 Gene ID (NCBI): 7178 RRID: AB_2882064 Conjugate: Unconjugated Form: Liquid Purification Method: Protein G purification UNIPROT ID: P13693 Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20 o C storage. 20ul sizes contain 0.1% BSA.