Proteintech, 66734-1-Ig, TDP-43 Monoclonal antibody

Size: 20ul / 150ul The TDP-43 (66734-1-Ig) by Proteintech is a Monoclonal antibody targeting TDP-43 in WB, IHC, ELISA applications with reactivity to human, mouse, rat samples 66734-1-Ig targets TDP-43 in WB, IHC, ELISA applications and shows reactivity with human, mouse, rat samples. Tested Applications Positive WB detected in: HeLa cells, HEK-293 cells, Jurkat cells, HSC-T6 cells, C6 cells, NIH/3T3 cells Positive IHC detected in: mouse brain tissue, human gliomas tissue, rat liver tissueNote: suggested antigen retrieval withTE buffer pH 9.0;(*) Alternatively, antigen retrieval may be performed withcitrate buffer pH 6.0 Recommended dilution Western Blot (WB): WB : 1:5000-1:50000 Immunohistochemistry (IHC): IHC : 1:1000-1:4000 Background Information Transactivation response (TAR), DNA-binding protein of 43 kDa (also known as TARDBP or TDP-43), was first isolated as a transcriptional inactivator binding to the TAR DNA element of the HIV-1 virus. Neumann et al. (2006) found that a hyperphosphorylated, ubiquitinated, and cleaved form of TARDBP, known as pathologic TDP-43, is the major component of the tau-negative and ubiquitin-positive inclusions that characterize amyotrophic lateral sclerosis (ALS) and the most common pathological subtype of frontotemporal lobar degeneration (FTLD-U). 66734-1-Ig is a mouse monoclonal antibody recognizing the intact protein of TDP-43. Specification Tested Reactivity: human, mouse, rat Cited Reactivity: human, mouse Host / Isotype: Mouse / IgG1 Class: Monoclonal Type: Antibody Immunogen: Recombinant protein Predict reactive species Full Name: TAR DNA binding protein Calculated Molecular Weight: 43 kDa Observed Molecular Weight: 43 kDa GenBank Accession Number: BC001487 Gene Symbol: TDP-43 Gene ID (NCBI): 23435 RRID: AB_2882084 Conjugate: Unconjugated Form: Liquid Purification Method: Protein G purification UNIPROT ID: Q13148 Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20 o C storage. 20ul sizes contain 0.1% BSA.