Proteintech, 66754-1-Ig, TNFRSF21/DR6 Monoclonal antibody

Size: 20ul / 150ul The TNFRSF21/DR6 (66754-1-Ig) by Proteintech is a Monoclonal antibody targeting TNFRSF21/DR6 in WB, IF/ICC, ELISA applications with reactivity to human samples 66754-1-Ig targets TNFRSF21/DR6 in WB, IF/ICC, ELISA applications and shows reactivity with human samples. Tested Applications Positive WB detected in: K-562 cells, THP-1 cells, Jurkat cells, Raji cells, Daudi cells Positive IF/ICC detected in: HepG2 cells Recommended dilution Western Blot (WB): WB : 1:1000-1:6000 Immunofluorescence (IF)/ICC: IF/ICC : 1:400-1:1600 Background Information TNFRSF21 (also known as DR6 or CD358) is an orphan receptor of the tumor necrosis factor (TNF) receptor superfamily. It controls cell death and differentiation in a cell-autonomous manner in different cell types including neurons, oligodendrocytes, endothelial cells, and immune cells (PMID: 29459438). DR6 has been shown to activate NF-κB and JNK, and induce cell apoptosis (PMID: 9714541). DR6 interacts with TRADD, which is known to mediate signal transduction of TNF receptors. It has been reported that APP binds DR6 to trigger axon pruning and neuron death via distinct caspases, indicating that DR6 might be involved in Alzheimer's disease (PMID: 19225519). Specification Tested Reactivity: human Cited Reactivity: human Host / Isotype: Mouse / IgG1 Class: Monoclonal Type: Antibody Immunogen: CatNo: Ag14327 Product name: Recombinant human TNFRSF21 protein Source: e coli. -derived, T-HIS Tag: 6*His Domain: 365-655 aa of BC021572 Sequence: IVVCSIRKSSRTLKKGPRQDPSAIVEKAGLKKSMTPTQNREKWIYYCNGHGIDILKLVAAQVGSQWKDIYQFLCNASEREVAAFSNGYTADHERAYAALQHWTIRGPEASLAQLISALRQHRRNDVVEKIRGLMEDTTQLETDKLALPMSPSPLSPSPIPSPNAKLENSALLTVEPSPQDKNKGFFVDESEPLLRCDSTSSGSSALSRNGSFITKEKKDTVLRQVRLDPCDLQPIFDDMLHFLNPEELRVIEEIPQAEDKLDRLFEIIGVKSQEASQTLLDSVYSHLPDLL Predict reactive species Full Name: tumor necrosis factor receptor superfamily, member 21 Calculated Molecular Weight: 655 aa, 72 kDa Observed Molecular Weight: 69 kDa GenBank Accession Number: BC021572 Gene Symbol: DR6 Gene ID (NCBI): 27242 RRID: AB_2882100 Conjugate: Unconjugated Form: Liquid Purification Method: Protein G purification UNIPROT ID: O75509 Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20 o C storage. 20ul sizes contain 0.1% BSA.