Proteintech, 66755-1-Ig, TRAF4 Monoclonal antibody

Size: 20ul / 150ul The TRAF4 (66755-1-Ig) by Proteintech is a Monoclonal antibody targeting TRAF4 in WB, IHC, IF/ICC, ELISA applications with reactivity to human, pig samples 66755-1-Ig targets TRAF4 in WB, IHC, IF/ICC, ELISA applications and shows reactivity with human, pig samples. Tested Applications Positive WB detected in: Jurkat cells, HeLa cells, THP-1 cells, U-937 cells, NCCIT cells, human brain tissue, pig brain tissue, HEK-293 cells, Ramos cells, Daudi cells, Raji cells, pig spleen tissue Positive IHC detected in: human urothelial carcinoma tissue, human lung cancer tissue, human breast cancer tissueNote: suggested antigen retrieval withTE buffer pH 9.0;(*) Alternatively, antigen retrieval may be performed withcitrate buffer pH 6.0 Positive IF/ICC detected in: HeLa cells Recommended dilution Western Blot (WB): WB : 1:1000-1:6000 Immunohistochemistry (IHC): IHC : 1:200-1:800 Immunofluorescence (IF)/ICC: IF/ICC : 1:50-1:500 Background Information The tumor necrosis factor receptor-associated factor (TRAF) family, consisting of seven members (TRAF1-7), is a group of signaling adaptors which participate in various signaling pathways through binding of the tumor necrosis factor receptor superfamily. As one of the important member of TRAF family proteins, TRAF4 was initially isolated from breast carcinomas and identified as the first member of the TRAF family to be up-regulated in human carcinomas. TRAF4 is required during embryogenesis in key biological processes including the formation of the trachea, the development of the axial skeleton, and the closure of the neural tube. TRAF4 deficiency leads to severe developmental alterations. Specification Tested Reactivity: human, pig Cited Reactivity: human, mouse Host / Isotype: Mouse / IgG1 Class: Monoclonal Type: Antibody Immunogen: CatNo: Ag27221 Product name: Recombinant human TRAF4 protein Source: e coli. -derived, PET28a Tag: 6*His Domain: 35-307 aa of BC001769 Sequence: GHRFCDTCLQEFLSEGVFKCPEDQLPLDYAKIYPDPELEVQVLGLPIRCIHSEEGCRWSGPLRHLQGHLNTCSFNVIPCPNRCPMKLSRRDLPAHLQHDCPKRRLKCEFCGCDFSGEAYESHEGMCPQESVYCENKCGARMMRRLLAQHATSECPKRTQPCTYCTKEFVFDTIQSHQYQCPRLPVACPNQCGVGTVAREDLPGHLKDSCNTALVLCPFKDSGCKHRCPKLAMARHVEESVKPHLAMMCALVSRQRQELQELRRELEELSVGSD Predict reactive species Full Name: TNF receptor-associated factor 4 Calculated Molecular Weight: 54 kDa Observed Molecular Weight: 50-54 kDa GenBank Accession Number: BC001769 Gene Symbol: TRAF4 Gene ID (NCBI): 9618 RRID: AB_2882101 Conjugate: Unconjugated Form: Liquid Purification Method: Protein G purification UNIPROT ID: Q9BUZ4 Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20 o C storage. 20ul sizes contain 0.1% BSA.