Proteintech, 66782-1-Ig, ROCK1 Monoclonal antibody

Size: 20ul / 150ul The ROCK1 (66782-1-Ig) by Proteintech is a Monoclonal antibody targeting ROCK1 in WB, ELISA applications with reactivity to human, mouse, rat samples 66782-1-Ig targets ROCK1 in WB, IHC, ELISA applications and shows reactivity with human, mouse, rat samples. Tested Applications Positive WB detected in: HeLa cells, HEK-293 cells, HepG2 cells, Jurkat cells, HSC-T6 cells, NIH/3T3 cells, RAW 264.7 cells Recommended dilution Western Blot (WB): WB : 1:1000-1:4000 Background Information ROCK1, also named as p160ROCK and NY-REN-35, belongs to the protein kinase superfamily and AGC Ser/Thr protein kinase family. ROCK1 phosphorylates and activates DAPK3, which then regulates myosin light chain phosphatase through phosphorylation of MYPT1 thereby regulating the assembly of the actin cytoskeleton, cell migration, invasiveness of tumor cells, smooth muscle contraction and neurite outgrowth. ROCK1 is required for centromere positioning and centromere-dependent exit from mitosis. It is necessary for apoptotic membrane blebbing. ROCK1 catalyzes the reaction: ATP + a protein = ADP + a phosphoprotein. Specification Tested Reactivity: human, mouse, rat Cited Reactivity: human, mouse Host / Isotype: Mouse / IgG1 Class: Monoclonal Type: Antibody Immunogen: CatNo: Ag16317 Product name: Recombinant human ROCK1 protein Source: e coli. -derived, PGEX-4T Tag: GST Domain: 401-710 aa of BC113114 Sequence: SNRRYLSSANPNDNRTSSNADKSLQESLQKTIYKLEEQLHNEMQLKDEMEQKCRTSNIKLDKIMKELDEEGNQRRNLESTVSQIEKEKMLLQHRINEYQRKAEQENEKRRNVENEVSTLKNQLEDLKKVSQNSQLANEKLSQLQKQLEEANDLLRTESDTAVRLRKSHTEMSKSISQLESLNRELQERNRILENSKSQTDKDYYQLQAILEAERRDRGHDSEMIGDLQARITSLQEEVKHLKHNLEKVEGERKEAQDMLNHSEKEKNNLEIDLNYKLKSLQQRLEQEVNEHKVTKARLTDKHQSIEEAKS Predict reactive species Full Name: Rho-associated, coiled-coil containing protein kinase 1 Calculated Molecular Weight: 1354 aa, 158 kDa Observed Molecular Weight: 150-160 kDa GenBank Accession Number: BC113114 Gene Symbol: ROCK1 Gene ID (NCBI): 6093 RRID: AB_2882127 Conjugate: Unconjugated Form: Liquid Purification Method: Protein G purification UNIPROT ID: Q13464 Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20 o C storage. 20ul sizes contain 0.1% BSA.