Proteintech, 66814-1-Ig, CEP250,C-NAP1 Monoclonal antibody

Size: 20ul / 150ul The CEP250,C-NAP1 (66814-1-Ig) by Proteintech is a Monoclonal antibody targeting CEP250,C-NAP1 in WB, IF/ICC, ELISA applications with reactivity to human samples 66814-1-Ig targets CEP250,C-NAP1 in WB, IHC, IF/ICC, ELISA applications and shows reactivity with human samples. Tested Applications Positive WB detected in: HeLa cells, HEK-293 cells, HepG2 cells, Jurkat cells, K-562 cells, HL-60 cells Positive IF/ICC detected in: HepG2 cells Recommended dilution Western Blot (WB): WB : 1:1000-1:8000 Immunofluorescence (IF)/ICC: IF/ICC : 1:400-1:1500 Background Information CEP250, also known as C-Nap1, is a coiled-coil protein that localizes to the proximal ends of mother and daughter centrioles. It is required for centriole-centriole cohesion during interphase of the cell cycle. It dissociates from the centrosomes when parental centrioles separate at the beginning of mitosis. The protein associates with and is phosphorylated by NIMA-related kinase 2, which is also associated with the centrosome. Specification Tested Reactivity: human Cited Reactivity: human, mouse Host / Isotype: Mouse / IgG1 Class: Monoclonal Type: Antibody Immunogen: CatNo: Ag5925 Product name: Recombinant human CEP250,C-NAP1 protein Source: e coli. -derived, PET28a Tag: 6*His Domain: 40-293 aa of BC001433 Sequence: RKLKNSQEAQQRQATLVRKLQAKVLQYRSWCQELEKRLEATGGPIPQRWENVEEPNLDELLVRLEEEQQRCESLAEVNTQLRLHMEKADVVNKALREDVEKLTVDWSRARDELMRKESQWQMEQEFFKGYLKGEHGRLLSLWREVVTFRRHFLEMKSATDRDLMELKAEHVRLSGSLLTCCLRLTVGAQSREPNGSGRMDGREPAQLLLLLAKTQELEKEAHERSQELIQLKSQGDLEKAELQDRVTELSALLT Predict reactive species Full Name: centrosomal protein 250kDa Calculated Molecular Weight: 281 kDa Observed Molecular Weight: 280 kDa GenBank Accession Number: BC001433 Gene Symbol: CEP250/CNAP1 Gene ID (NCBI): 11190 RRID: AB_2882157 Conjugate: Unconjugated Form: Liquid Purification Method: Protein G purification UNIPROT ID: Q9BV73 Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20 o C storage. 20ul sizes contain 0.1% BSA.